Harry Butler Institute, Murdoch University, Perth 6150, Australia.
Indian Council of Agricultural Research─Indian Grassland and Fodder Research Institute, Jhansi 284003, India.
J Agric Food Chem. 2024 Nov 13;72(45):24944-24952. doi: 10.1021/acs.jafc.4c03986. Epub 2024 Nov 1.
Direct immersion solid-phase microextraction coupled with gas chromatography-mass spectroscopy was used to create chemical fingerprints of annual ryegrass cultivars (). Extracts made of the inflorescences of four cultivars and one accession of annual ryegrass were assessed to identify differential metabolites between those resistant to and susceptible to bacterial galls associated with annual ryegrass toxicity (ARGT). Numerous compounds were identified. Principal component analysis showed distinct clustering of metabolites from disease-resistant and disease-susceptible cultivars. Partial least-squares-discriminant analysis identified sterols, esters, aldehydes, and terpenes that correlated with resistance to galls formation. Esters, sterols, phenols, heterocyclics, fatty acids, organofluorides, and siloxanes were predominant in resistant genotypes, whereas alcohols, aldehydes, terpenes, and hydrocarbons were predominant in susceptible genotypes. The identification of differentially expressed metabolites provides potential chemical markers to guide breeding strategies for ARGT resistance in ryegrass.
直接浸入式固相微萃取结合气相色谱-质谱联用技术用于创建一年生黑麦草品种的化学指纹图谱。对四个品种和一个一年生黑麦草品系的花序提取物进行评估，以鉴定与一年生黑麦草毒性相关的细菌性肿瘤（ARGT）抗性和易感品种之间的差异代谢物。鉴定出许多化合物。主成分分析显示，来自抗病和感病品种的代谢物明显聚类。偏最小二乘判别分析确定了与抗肿瘤形成相关的甾醇、酯、醛和萜烯。酯类、甾醇类、酚类、杂环类、脂肪酸类、有机氟化物和硅氧烷类在抗性基因型中占优势，而醇类、醛类、萜烯类和烃类在易感基因型中占优势。差异表达代谢物的鉴定为指导黑麦草 ARGT 抗性的育种策略提供了潜在的化学标记。
