Department of Endocrinology, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 211198, China.
Mudi Meng Honors College, China Pharmaceutical University, Nanjing, 211198, China.
Cell Commun Signal. 2024 Nov 1;22(1):530. doi: 10.1186/s12964-024-01908-z.
Papillary thyroid carcinoma (PTC) is the most common type of thyroid malignancy, with a rising incidence. Traditional treatments, such as thyroidectomy and radiotherapy, often lead to significant side effects, including impaired thyroid function. Therefore, there is an urgent need for non-invasive therapeutic approaches. This study aims to explore the potential of photobiomodulation therapy (PBMT), a non-invasive treatment using specific wavelengths of light, in the management of PTC.
We investigated the effects of blue light PBMT on PTC cells, focusing on the Retinal-OPSIN 3 (OPN3) complex's role in mediating cellular responses. Blue light exposure was applied to PTC cells, and subsequent changes in cellular proliferation, cell cycle progression, and protein expression were analyzed. Statistical tests, including one-way ANOVA and t-tests, were used to evaluate the significance of the findings.
Blue light exposure led to the dissociation of 11-cis-retinal from OPN3, resulting in the accumulation of all-trans retinal. This accumulation disrupted cellular proliferation pathways and induced G0/G1 cell cycle arrest in PTC cells. The Retinal-OPN3 complex was found to be a key mediator in these processes, demonstrating that thyroid cells can respond to specific light wavelengths and utilize their photoreceptive potential for therapeutic purposes.
Our findings suggest that PBMT, through the modulation of the Retinal-OPN3 complex, offers a promising non-invasive approach for treating PTC. This study highlights the therapeutic potential of light signal transduction in non-ocular tissues and opens new avenues for non-invasive cancer therapies.
甲状腺癌（PTC）是最常见的甲状腺恶性肿瘤，发病率呈上升趋势。传统的治疗方法，如甲状腺切除术和放射治疗，往往会导致显著的副作用，包括甲状腺功能受损。因此，迫切需要非侵入性的治疗方法。本研究旨在探讨光生物调节疗法（PBMT）的潜力，这是一种使用特定波长光的非侵入性治疗方法，用于治疗 PTC。
我们研究了蓝光 PBMT 对 PTC 细胞的影响，重点研究了视网膜-OPSIN 3（OPN3）复合物在介导细胞反应中的作用。将蓝光暴露应用于 PTC 细胞，分析细胞增殖、细胞周期进程和蛋白质表达的后续变化。使用单因素方差分析和 t 检验等统计检验来评估研究结果的显著性。
蓝光暴露导致 11-顺式视黄醛与 OPN3 分离，导致全反式视黄醛积累。这种积累破坏了细胞增殖途径，并诱导 PTC 细胞中的 G0/G1 细胞周期停滞。视网膜-OPN3 复合物被发现是这些过程中的关键介质，表明甲状腺细胞可以对特定的光波长做出反应，并利用其光感受潜能进行治疗。
我们的研究结果表明，PBMT 通过调节视网膜-OPN3 复合物，为治疗 PTC 提供了一种有前途的非侵入性方法。本研究强调了光信号转导在非眼组织中的治疗潜力，并为非侵入性癌症治疗开辟了新途径。
