School of Ecology and Environment, Northwestern Polytechnical University, Xi'an, 710072, China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, 224002, China.
Sci Data. 2024 Nov 2;11(1):1190. doi: 10.1038/s41597-024-04031-3.
Land hermit crabs are a group of shell-carrying crabs that have evolved remarkable terrestrial adaptations in behavior, morphology, physiology, and biochemistry. However, the genetic mechanisms underlying these adaptations remain unclear. In addition, usually it is very difficult to get good genome assemblies for crustaceans. In this study, we managed to assemble the first chromosome-level genome for a land hermit crab (Coenobita brevimanus) with careful manual curation. The final assembly spans 4.74 Gb, with the contig N50 of 1.75 Mb and scaffold N50 of 42.95 Mb, encompassing 117 chromosomes that account for 96.54% of the genome. The evaluations including genome BUSCO (95.26%), Merqury qv (35.88) and the mapping ratio of pair-end short reads (99.48%) showed the high-continuity of C. brevimanus genome assembly, making it the genome with the highest quality in crustaceans with genome size bigger than 3 Gb. The availability of this chromosome-scale genome of crustaceans represents a valuable resource for the land hermit crab, which represents an independent water-to-land transition evolutionary event in the animal kingdom.
地寄居蟹是一群具有非凡陆地适应能力的带壳蟹类，它们在行为、形态、生理和生物化学方面都有进化。然而，这些适应的遗传机制尚不清楚。此外，通常情况下，甲壳类动物的基因组组装质量很难得到保证。在这项研究中，我们通过仔细的人工编辑，成功组装了第一个地寄居蟹（ Coenobita brevimanus ）的染色体水平基因组。最终组装的基因组大小为 4.74Gb，其 contig N50 为 1.75Mb， scaffold N50 为 42.95Mb，包含 117 条染色体，占基因组的 96.54%。基因组 BUSCO（95.26%）、Merqury qv（35.88%）和双端短读测序的比对率（99.48%）的评估表明，C. brevimanus 基因组组装具有高度的连续性，使其成为基因组大小大于 3Gb 的甲壳类动物中质量最高的基因组。该甲壳类动物染色体水平基因组的可用性为地寄居蟹提供了一个有价值的资源，它代表了动物王国中一个独立的水到陆的过渡进化事件。
