Einstein Center for Neurosciences, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Université Paris Cité, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Paris, France.
Nat Commun. 2024 Nov 5;15(1):9555. doi: 10.1038/s41467-024-53685-5.
Voltage imaging holds great potential for biomedical research by enabling noninvasive recording of the electrical activity of excitable cells such as neurons or cardiomyocytes. Camera-based detection can record from hundreds of cells in parallel, but imaging entire volumes is limited by the need to focus through the sample at high speeds. Remote focusing techniques can remedy this drawback, but have so far been either too slow or light-inefficient. Here, we introduce flipped image remote focusing, a remote focusing method that doubles the light efficiency compared to conventional beamsplitter-based techniques and enables high-speed volumetric voltage imaging at 500 volumes/s. We show the potential of our approach by combining it with light sheet imaging in the zebrafish spinal cord to record from >100 spontaneously active neurons in parallel.
电压成像是生物医学研究的一个重要工具，它可以实现对神经元或心肌细胞等可兴奋细胞的电活动进行非侵入式记录。基于相机的检测可以同时记录数百个细胞的信号，但要对整个样本进行成像则需要高速穿透样本进行聚焦，这成为了一个限制因素。远程聚焦技术可以解决这个问题，但迄今为止，这些技术要么速度太慢，要么效率太低。在这里，我们介绍了翻转图像远程聚焦技术，与传统的分束器技术相比，这种新技术提高了两倍的光效率，能够以 500 体/秒的速度实现高速容积电压成像。我们通过将其与斑马鱼脊髓中的光片成像相结合，对超过 100 个自发活跃的神经元进行并行记录，展示了该方法的应用潜力。
