在坦桑尼亚疑似牛结节疹疫情爆发中检测和分子特征分析牛结节疹病毒和牛丘疹性口炎病毒。

Detection and molecular characterization of lumpy skin disease and bovine papular stomatitis viruses in lumpy skin disease-suspected outbreaks in Tanzania.

机构信息

Tanzania Veterinary Laboratory Agency, P.O.BOX 9254, Dar es salaam, Tanzania.

Animal Production and Health Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, Vienna, A-1400, Austria.

出版信息

Virol J. 2024 Nov 5;21(1):276. doi: 10.1186/s12985-024-02558-w.

DOI:10.1186/s12985-024-02558-w

PMID:39501408

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11539547/

Abstract

BACKGROUND

Lumpy Skin Disease (LSD) is endemic in sub-Saharan countries and is currently a global threat to the cattle industry. Information on the circulating Capripoxvirus lumpyskinpox, formerly known as Lumpy Skin Disease Virus (LSDV), and other poxviruses infecting cattle is very scant in Tanzania. The current study aimed to confirm and characterize LSDV and other poxviruses infecting cattle, from LSD suspected outbreaks in Tanzania.

METHODS

A total of 24 samples were collected from four LSD suspected outbreaks reported in Tanzania between February and May 2023. Samples were screened for LSDV genome by real-time PCR and then subjected to a high-resolution multiplex melting (HRM) assay where 10 samples were positive for Capripoxvirus (CaPV) and one sample was Parapoxvirus (PPV) positive. Four LSDV genes; RPO30, GPCR, EEV glycoprotein and B22R and the partial B2L gene of PPVs were analyzed.

RESULTS

All targeted LSDV genes from the Tanzanian isolates showed 100% similarity and isolates clustered with commonly circulating LSDV field isolates. Furthermore, the single nucleotide polymorphism (SNP) at position 240 (A-> G) of the EEV gene differentiates the Tanzanian LSDVs from the group of ancient Kenyan LSDV isolates while the B22R sequences of the Tanzanian LSDV isolates differed from the LSDV Neethling and LSDV KSGP-0240 derived vaccines. Sequence analysis of the partial B2L gene of the Tanzanian parapoxvirus bovinestomatitis, formerly known as Bovine papular stomatitis virus (BPSV) showed a different BPSV strain circulating compared to publicly available sequences.

CONCLUSION

These findings confirm the presence of LSDV in Tanzania, which suggesting the need for establishing an effective control program and continuous monitoring. The presence of a typical profile for Tanzania BPSV is an indication that, although never reported before, BPSV is established in the country therefore this virus should be included in the differential diagnosis of LSDV.

摘要

背景

块状皮肤病（LSD）在撒哈拉以南国家流行，目前对全球牛养殖业构成威胁。坦桑尼亚关于流行的羊痘病毒（以前称为 LSD 病毒）和其他感染牛的痘病毒的信息非常匮乏。本研究旨在确认和描述来自坦桑尼亚 LSD 疑似暴发的 LSDV 和其他牛痘病毒。

方法

2023 年 2 月至 5 月期间，共从坦桑尼亚报告的四起 LSD 疑似暴发中采集了 24 份样本。通过实时 PCR 筛选 LSDV 基因组，然后进行高分辨率多重熔解（HRM）检测，其中 10 份样本为羊痘病毒（CaPV）阳性，1 份样本为副牛痘病毒（PPV）阳性。分析了 LSDV 的 4 个基因；RPO30、GPCR、EEV 糖蛋白和 B22R 以及 PPV 的部分 B2L 基因。

结果

来自坦桑尼亚分离株的所有 LSDV 目标基因均显示 100%的相似性，分离株与常见的 LSDV 田间分离株聚集在一起。此外，EEV 基因位置 240 （A->G）的单核苷酸多态性（SNP）将坦桑尼亚 LSDV 与肯尼亚古老 LSDV 分离株区分开来，而来自坦桑尼亚 LSDV 分离株的 B22R 序列与 LSDV Neethling 和 LSDV KSGP-0240 衍生疫苗不同。坦桑尼亚牛传染性脓疱性皮炎，以前称为牛口炎病毒（BPSV）的部分 B2L 基因序列分析显示，与公开可用序列相比，循环的 BPSV 株不同。