Department of Neurosurgery, The Affiliated Hospital of Xuzhou Medical University, West Huai-Hai Road 99, Xuzhou, Jiangsu, China.
Department of Neurosurgery, The Affiliated Huai'an Hospital of Xuzhou Medical University, South Huai-Hai Road 62, Huai'an, Jiangsu, China.
Mediators Inflamm. 2024 Oct 29;2024:6147483. doi: 10.1155/2024/6147483. eCollection 2024.
Low-grade glioma (LGG) is a commonly occurring type of central nervous system cancer. Integrin α1 (ITGA1), a family member of integrins, is implied in the malignant development of cancers, but the fundamental role of ITGA1 has not been illustrated yet in glioma. This study aimed to evaluate the prognostic value of ITGA1. Correlations between ITGA1 and relevant clinical features were analyzed in the LGG datasets based on Chinese Glioma Genome Atlas (CGGA) and Tumor Genome Atlas (TCGA). Glioma clinical samples and glioma cell lines were identified at the level of mRNA and protein level by Western blot. Cox regression were developed to assess the involvement of ITGA1 expression in predicting survival in LGG patients. Application of GSEA enrichment analysis to reveal ITGA1-mediated biological functions in LGG. Using TIMER 2.0 to analyze correlations between immune cell infiltration. In addition, ITGA1 high expression was analyzed for correlation with immune checkpoint-related genes and cumulative survival time. ITGA1 was significantly more expressed in LGG than in normal samples. Cox regression indicated that ITGA1 was a risk factor independently for prognosis in LGG patients. GSEA enrichment analysis indicated that ITGA1 was engaged in several immunomodulatory processes. ITGA1 expression was shown to be highly correlated with the immune score, stromal score, and estimate score in LGG. ITGA1 was significantly related to the immune checkpoint-associated gene expression. In vivo experiments showed that overexpression of ITGA1 promoted glioma cell invasion. High ITGA1 expression is correlated with immune infiltration of the low-grade tumor, leading to poor prognoses in LGG patients.
低级别胶质瘤 (LGG) 是一种常见的中枢神经系统癌症。整合素 α1 (ITGA1) 是整合素家族的一员，它参与了癌症的恶性发展，但在胶质瘤中，ITGA1 的基本作用尚未阐明。本研究旨在评估 ITGA1 的预后价值。通过中国脑胶质瘤基因组图谱 (CGGA) 和肿瘤基因组图谱 (TCGA) 分析了 LGG 数据集，分析了 ITGA1 与相关临床特征的相关性。通过 Western blot 在mRNA 和蛋白水平上鉴定了胶质瘤临床样本和胶质瘤细胞系。采用 Cox 回归评估 ITGA1 表达对预测 LGG 患者生存的影响。应用 GSEA 富集分析揭示 ITGA1 在 LGG 中的介导的生物学功能。使用 TIMER 2.0 分析免疫细胞浸润的相关性。此外，还分析了 ITGA1 高表达与免疫检查点相关基因和累积生存时间的相关性。ITGA1 在 LGG 中表达明显高于正常样本。Cox 回归表明 ITGA1 是 LGG 患者独立预后的危险因素。GSEA 富集分析表明，ITGA1 参与了几个免疫调节过程。ITGA1 的表达与 LGG 中的免疫评分、基质评分和估计评分高度相关。ITGA1 与免疫检查点相关基因的表达显著相关。体内实验表明，ITGA1 的过表达促进了胶质瘤细胞的侵袭。高 ITGA1 表达与低级别肿瘤的免疫浸润相关，导致 LGG 患者预后不良。
