Chair and Department of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, 20-081 Lublin, Poland.
Body Composition Research Laboratory, Department of Preclinical Science, Medical University of Lublin, 20-059 Lublin, Poland.
Cells. 2024 Oct 23;13(21):1753. doi: 10.3390/cells13211753.
Metagenomic research has allowed the identification of numerous viruses present in the human body. Viruses may significantly increase the likelihood of developing intrauterine fetal growth restriction (FGR). The goal of this study was to examine and compare the virome of normal and FGR placentas using proteomic techniques.
The study group of 18 women with late FGR was compared with 18 control patients with physiological pregnancy and eutrophic fetus. Proteins from the collected afterbirth placentas were isolated and examined using liquid chromatography linked to a mass spectrometer.
In this study, a group of 107 viral proteins were detected compared to 346 in the controls. In total, 41 proteins were common in both groups. In total, 64 proteins occurred only in the study group and indicated the presence of bacterial phages: , , , , , , and amoebae , , , , , , pol proteins of virus, and proteins of , , and heat shock proteins of the virus Out of 297 proteins found only in the control group, only 2 viral proteins occurred statistically significantly more frequently: 1/ [uncultured phage uvMED] and VP4 [ WZ-2015a].
The detection of certain viral proteins exclusively in the control group suggests that they may play a protective role. Likewise, the proteins identified only in the study group could indicate a potentially pathogenic function. A virome study may be used to identify an early infection, evaluate its progress, and possible association with fetal growth restriction. Utilizing this technology, an individualized patient therapy is forthcoming, e.g., vaccines.
宏基因组学研究已经鉴定了人体中存在的许多病毒。病毒可能会显著增加胎儿宫内生长受限（FGR）的发生几率。本研究的目的是使用蛋白质组学技术检测和比较正常和 FGR 胎盘的病毒组。
本研究将 18 例晚期 FGR 患者与 18 例生理性妊娠和胎儿营养良好的对照患者进行了比较。从收集的胎盘中分离蛋白质，并使用液相色谱-质谱联用进行检查。
与对照组的 346 种蛋白质相比，本研究中检测到 107 种病毒蛋白。总共有 41 种蛋白质在两组中均存在。总共有 64 种蛋白质仅在研究组中出现，表明存在细菌噬菌体：、、、、、、和变形虫、、、、、、病毒的 pol 蛋白和、和病毒的热休克蛋白。在仅在对照组中发现的 297 种蛋白质中，只有 2 种病毒蛋白的出现具有统计学意义：1/[未培养噬菌体 uvMED]和 VP4[WZ-2015a]。
仅在对照组中检测到某些病毒蛋白表明它们可能具有保护作用。同样，仅在研究组中鉴定出的蛋白质可能表明具有潜在的致病功能。病毒组学研究可用于识别早期感染、评估其进展以及与胎儿生长受限的可能关联。利用这项技术，即将开展针对患者的个体化治疗，例如疫苗。
