Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia.
Koltzov Institute of Development Biology of Russia Academy of Science, 119334 Moscow, Russia.
Cells. 2024 Oct 23;13(21):1757. doi: 10.3390/cells13211757.
Scanning probe microscopy (SPM) is a versatile tool for studying a wide range of materials. It is well suited for investigating living matter, for example, in single-cell neutrophil studies. SPM has been extensively utilized to analyze cell physical properties, providing detailed insights into their structural and functional characteristics at the nanoscale. Its long-standing application in this field highlights its essential role in cell biology and immunology research, significantly contributing to understanding cellular mechanics and interactions. In this review, we discuss the application of SPM techniques, specifically atomic force microscopy (AFM) and scanning ion-conductance microscopy (SICM), to study the fundamental functions of neutrophils. In addition, recent advances in the application of SPM in single-cell immunology are discussed. The application of these techniques allows for obtaining data on the morphology, topography, and mechanical and electrochemical properties of neutrophils with high accuracy.
扫描探针显微镜（SPM）是一种功能强大的工具，可用于研究广泛的材料。它非常适合研究生物物质，例如单细胞中性粒细胞的研究。SPM 已被广泛用于分析细胞的物理特性，为纳米尺度上的结构和功能特征提供了详细的见解。它在该领域的长期应用凸显了其在细胞生物学和免疫学研究中的重要作用，对理解细胞力学和相互作用做出了重要贡献。在这篇综述中，我们讨论了 SPM 技术（特别是原子力显微镜（AFM）和扫描离子传导显微镜（SICM））在研究中性粒细胞基本功能方面的应用。此外，还讨论了 SPM 在单细胞免疫学中的应用的最新进展。这些技术的应用可以非常精确地获取有关中性粒细胞形态、形貌、机械和电化学特性的数据。
