LG Household and Health Care R&D Center, Seoul 07795, Republic of Korea.
Department of Natural Medicine, School of Pharmacy, Fudan University, Shanghai 201203, China.
Cells. 2024 Oct 30;13(21):1799. doi: 10.3390/cells13211799.
Nicotinamide adenine dinucleotide (NAD) is indispensable for the regulation of biological metabolism. Previous studies have revealed its role in aging and degenerative diseases, while crucially showing that supplementation with NAD or its precursors could ameliorate or reverse the progression of aging. Despite extensive evidence for the role and action of NAD in aging, its pharmacological activity on the skin, or even its mechanism, has not been elucidated. In this study, we established a novel approach to effectively utilize NAD for skin anti-aging by enhancing the pharmacological efficacy of exogenous NAD using a phytochemical complex consisting of quercetin, and enoxolone through inhibition of CD38. Through the comprehensive in vitro experiments based on human fibroblasts, we observed that exogenous NAD could exert protective effects against both extrinsic aging induced by ultraviolet light exposure and intrinsic aging. Additionally, we found that its effects were significantly boosted by quercetin and enoxolone. In this in-depth study, we demonstrated that these beneficial effects are mediated by improved sirtuin activation, autophagy, and mitochondrial functionality. Our approach is expected to verify the applicability of the topical application of NAD and offer more effective solutions for the unmet needs of patients and consumers who demand more effective anti-aging effects.
烟酰胺腺嘌呤二核苷酸（NAD）是调节生物代谢所必需的。先前的研究表明其在衰老和退行性疾病中的作用，同时关键表明补充 NAD 或其前体可以改善或逆转衰老的进程。尽管有大量证据表明 NAD 在衰老中的作用和作用，但它在皮肤中的药理学活性，甚至其机制，尚未阐明。在这项研究中，我们通过使用包含槲皮素和依诺酮的植物化学复合物抑制 CD38 来增强外源性 NAD 的药理功效，建立了一种有效利用 NAD 进行皮肤抗衰老的新方法。通过基于人成纤维细胞的综合体外实验，我们观察到外源性 NAD 可以对抗紫外线照射引起的外在衰老和内在衰老产生保护作用。此外，我们发现槲皮素和依诺酮显著增强了其作用。在这项深入的研究中，我们证明这些有益的作用是通过改善的沉默调节蛋白激活、自噬和线粒体功能来介导的。我们的方法有望验证 NAD 的局部应用的适用性，并为患者和消费者对更有效抗衰老效果的未满足需求提供更有效的解决方案。
