Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland.
Department of Infectious, Invasive Diseases and Veterinary Administration, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland.
Int J Mol Sci. 2024 Oct 23;25(21):11402. doi: 10.3390/ijms252111402.
Nano-priming is a relatively new seed treatment technique using metal and metal oxide nanoparticles (NPs), and such application of NPs may support the plants' immunity. Recently we have shown that the that biologically synthesized silver nanoparticles (bio-AgNPs) used as short-term foliar treatment protect pea seedlings against and . In the present study, the protection of peas against both fungal pathogens via seed priming with bio-AgNPs was analyzed. Moreover, the changes in the polar metabolic profiles of the seedlings caused by priming and infection were also compared. Seed priming with bio-AgNPs at concentrations of 50 and 100 mg/L considerably reduced the symptoms and infection levels of both pathogens by over 70% and 90% for and , respectively. Pathogens infection and nano-priming affected the metabolic profile of pea seedlings. The major changes in the primary metabolism were observed among carbohydrates and amino acids. In turn, this may result in changes in the expression and accumulation of secondary metabolites. Therefore, further investigation of the effect of nano-priming should focus on the changes in the secondary metabolism.
纳米引发是一种相对较新的种子处理技术，使用金属和金属氧化物纳米颗粒（NPs），并且这种 NPs 的应用可能支持植物的免疫。最近，我们已经表明，作为短期叶面处理的生物合成银纳米颗粒（bio-AgNPs）可保护豌豆幼苗免受 和 。在本研究中，通过用 bio-AgNPs 进行种子引发来分析豌豆对这两种真菌病原体的保护作用。此外，还比较了引发和感染引起的幼苗极性代谢谱的变化。用 50 和 100 mg/L 的 bio-AgNPs 进行种子引发可使两种病原体的症状和感染水平分别降低超过 70%和 90%，分别为 和 。病原体感染和纳米引发会影响豌豆幼苗的代谢谱。在碳水化合物和氨基酸中观察到主要的初级代谢变化。反过来，这可能导致次生代谢物的表达和积累发生变化。因此，纳米引发的效果的进一步研究应集中在次生代谢物的变化上。
