Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan.
Department of Molecular Genetics, Institute of Biomedical Science, Kansai Medical University, 2-5-1 Shinmachi, Hirakata, Osaka 573-1010, Japan.
Int J Mol Sci. 2024 Oct 24;25(21):11410. doi: 10.3390/ijms252111410.
This study aimed to test the effectiveness of Raman spectroscopy in the characterization of the degrees of physiological stress and virulence in clinical swab samples collected from patients affected by oral candidiasis. Raman experiments were conducted on a series of eight isolates, both in an as-collected state and after biofilm purification followed by 3 days of culture. The outputs were matched to optical microscopy observations and the results of conventional chromogenic medium assays. A statistically significant series of ten Raman spectra were collected for each clinical sample, and their averages were examined and interpreted as multiomic snapshots for and non- species. Spectroscopic analyses based on selected Raman parameters previously developed for standard samples revealed an extreme structural complexity for all of the clinical samples, which arose from the concurrent presence of a variety of biofilms and commensal bacteria in the samples, as well as a number of other biochemical circumstances affecting the cells in their physiological stress state. However, three Raman algorithms survived such complexity, which enabled insightful classifications of cells from clinical samples, in terms of their physiological stress and morphogenic state, membrane permeability, and virulence. These three characteristics, in turn, converged into a seemingly "fight or flight" response of the cells. Although yet preliminary, the present study points out criticalities and proposes solutions regarding the potential utility of Raman spectroscopy in fast bedside analyses of surveillance samples.
本研究旨在测试拉曼光谱在表征临床拭子样本中口腔念珠菌病患者生理应激和毒力程度方面的有效性。对八组分离物进行了一系列拉曼实验，包括在收集状态下以及生物膜纯化后培养 3 天后。将结果与光学显微镜观察和传统显色培养基检测结果相匹配。对每个临床样本采集了一系列具有统计学意义的十张拉曼光谱，对其平均光谱进行了检查和解释，作为 和非 种的多组学快照。基于先前为标准 样本开发的选定拉曼参数的光谱分析显示，所有临床样本都具有极高的结构复杂性，这是由于样本中存在多种生物膜和共生细菌，以及其他一些影响细胞生理应激状态的生化情况共同作用的结果。然而，有三种拉曼算法能够克服这种复杂性，能够深入了解临床样本中 细胞的生理应激和形态发生状态、膜通透性和毒力的分类。这三个特征反过来又汇聚成 细胞的一种似乎是“战斗或逃跑”的反应。尽管目前还处于初步阶段，但本研究指出了在快速床边分析监测样本中拉曼光谱的潜在应用的关键点，并提出了一些解决方案。
