Agricultural Research Service, Corn Insects, and Crop Genetics Research Unit, United States Department of Agriculture, Ames, IA 50010, USA.
Int J Mol Sci. 2024 Oct 25;25(21):11480. doi: 10.3390/ijms252111480.
Genes involved in iron deficiency responses have been well characterized in , but their roles in crop species have not been well explored. Reliance on model species may fail to identify novel iron stress mechanisms present within crop species, likely selected by hundreds of years of selection. Fiskeby III (PI 438471) is a soybean line from Sweden that demonstrates high levels of resilience to numerous stresses. Earlier Fiskeby III studies have identified a suite of genes responding to iron deficiency stress in Fiskeby III that are also associated with Arabidopsis iron deficiency responses. We were interested in determining how canonical iron genes function in Fiskeby III under normal and iron stress conditions. To investigate this, we used virus-induced gene silencing to knock down gene expression of three iron deficiency response genes (FER-like iron deficiency induced transcription factor (FIT), elongated hypocotyl 5 (HY5) and popeye (PYE)) in Fiskeby III. Analyses of RNAseq data generated from silenced plants in iron-sufficient and -deficient conditions found silencing FIT and HY5 altered general stress responses but did not impact iron deficiency tolerance, confirming Fiskeby III utilizes novel mechanisms to tolerate iron deficiency stress.
在 中，与缺铁反应相关的基因已经得到了很好的描述，但它们在作物物种中的作用尚未得到很好的探索。依赖模式物种可能无法识别作物物种中存在的新的铁胁迫机制，这些机制可能是经过数百年的选择而产生的。Fiskeby III（PI 438471）是来自瑞典的大豆品系，对多种胁迫具有很高的适应能力。早期的 Fiskeby III 研究已经确定了一套在 Fiskeby III 中对缺铁胁迫有反应的基因，这些基因也与拟南芥缺铁反应有关。我们有兴趣确定在正常和缺铁条件下，典型的铁基因在 Fiskeby III 中的作用。为了研究这一点，我们使用病毒诱导的基因沉默技术敲低了 Fiskeby III 中三个缺铁反应基因（FER-like iron deficiency induced transcription factor（FIT）、elongated hypocotyl 5（HY5）和popeye（PYE））的表达。对在铁充足和缺铁条件下沉默植物的 RNAseq 数据进行分析发现，沉默 FIT 和 HY5 改变了一般的应激反应，但不影响缺铁耐受性，这证实了 Fiskeby III 利用新的机制来耐受缺铁胁迫。
