Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81350-010, Brazil.
Post-Graduation Program in Genetics, Department of Genetics, Federal University of Parana, Curitiba 81530-980, Brazil.
Int J Mol Sci. 2024 Oct 26;25(21):11506. doi: 10.3390/ijms252111506.
Loop-mediated isothermal amplification (LAMP) is a highly effective molecular diagnostic technique, particularly advantageous for point-of-care (POC) settings. In recent years, LAMP has expanded to include various adaptations such as DARQ-LAMP, QUASR, FLOS-LAMP, displacement probes and molecular beacons. These methods enable multiplex detection of multiple targets in a single reaction, enhancing cost-effectiveness and diagnostic efficiency. Consequently, LAMP has gained significant traction in diagnosing diverse viruses, notably during the COVID-19 pandemic. However, its application for detecting Herpesviridae remains relatively unexplored. This group of viruses is of particular interest due to their latency and potential reactivation, crucial for immunocompromised patients, including organ and hematopoietic stem cell transplant recipients. This review highlights recent advancements in LAMP for virus diagnosis and explores current research trends and future prospects, emphasizing the detection challenges posed by Herpesviridae.
环介导等温扩增（LAMP）是一种高效的分子诊断技术，特别适用于即时检测（POC）环境。近年来，LAMP 已经扩展到包括各种适应方法，如 DARQ-LAMP、QUASR、FLOS-LAMP、置换探针和分子信标。这些方法能够在单个反应中同时检测多个目标，提高了成本效益和诊断效率。因此，LAMP 在诊断各种病毒方面取得了显著的进展，尤其是在 COVID-19 大流行期间。然而，它在检测疱疹病毒科方面的应用相对较少。由于疱疹病毒科潜伏和潜在的再激活，这些病毒尤其受到关注，对于免疫功能低下的患者，包括器官和造血干细胞移植受者至关重要。本综述强调了 LAMP 在病毒诊断方面的最新进展，并探讨了当前的研究趋势和未来前景，强调了疱疹病毒科检测所面临的挑战。
