Nantong Laboratory of Development and Diseases, School of Life Sciences, Co-Innovation Center of Neuroregeneration, Nantong University, Qixiu Road 19, Nantong 226001, China.
Int J Mol Sci. 2024 Oct 28;25(21):11561. doi: 10.3390/ijms252111561.
The development of sensory hair cells (HCs) is closely linked to hearing loss. There are still many unidentified genes that may play a crucial role in HC development and function. Glutamine synthetase, Glul, is expressed in sensory hair cells and auditory organs. However, the role of the gene family in the auditory system remains largely unexplored. This study aims to investigate the function of the gene family in the auditory system. The expression patterns of the gene family were examined via in situ hybridization in zebrafish embryos. It was revealed that the expression of occurred in the otic vesicle, while was expressed in the neuromast. In contrast, did not exhibit any discernible signal. loss of function caused abnormal otolith formation and reduced hair cell number in otic vesicles, while knockdown caused a decrease in HC number in both neuromasts and otic vesicles and impaired auditory function. Furthermore, we found that the knockdown of induces apoptosis of hair cells. Transcriptomic analysis of zebrafish with and knockdown revealed significant alterations in the expression of many genes associated with auditory organs. The current study sheds light on the requirement of and in zebrafish hair cell formation and auditory function.
感觉毛细胞（HCs）的发育与听力损失密切相关。仍有许多未被识别的基因可能在 HC 发育和功能中发挥关键作用。谷氨酰胺合成酶，Glul，在感觉毛细胞和听觉器官中表达。然而，该基因家族在听觉系统中的作用在很大程度上仍未被探索。本研究旨在研究该基因家族在听觉系统中的功能。通过原位杂交在斑马鱼胚胎中检查了该基因家族的表达模式。结果表明，在耳泡中表达，而在毛细胞中表达。相比之下，没有表现出任何明显的信号。功能丧失导致耳石形成异常和毛细胞数量减少，而敲低导致毛细胞数量减少在毛细胞和耳泡中，并且损害听觉功能。此外，我们发现的敲低诱导毛细胞凋亡。与 和 敲低的斑马鱼的转录组分析显示与听觉器官相关的许多基因的表达发生了显著改变。本研究阐明了在斑马鱼毛细胞形成和听觉功能中需要和。
