Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, College of Agriculture, Shihezi University, Shihezi 832000, China.
Int J Mol Sci. 2024 Oct 28;25(21):11575. doi: 10.3390/ijms252111575.
Carbon catabolite repression (CCR) is a common transcriptional regulatory mechanism that microorganisms use to efficiently utilize carbon nutrients, which is critical for the fitness of microorganisms and for pathogenic species to cause infection. Here, we characterized two CCR genes, and , in that cause cotton Verticillium wilt disease. The and knockout mutants displayed slow growth with decreased conidiation and microsclerotium production and reduced virulence to cotton, suggesting that and are involved in growth and pathogenicity in . We further generated 36 highly reliable and stable and libraries to comprehensively explore the dynamic expression of genes and metabolites when grown under different carbon sources and CCR conditions. Based on the weighted gene co-expression network analysis (WGCNA) and correlation networks, is co-expressed with a multitude of downregulated genes. These gene networks span multiple functional pathways, among which seven genes, including PYCR (pyrroline-5-carboxylate reductase), are potential target genes of . Different carbon source conditions triggered entirely distinct gene regulatory networks, yet they exhibited similar changes in metabolic pathways. Six genes, including 6-phosphogluconolactonase and 2-ODGH (2-oxoglutarate dehydrogenase E1), may serve as hub genes in this process. Both and could comprehensively influence the expression of plant cell wall-degrading enzyme (PCWDE) genes, suggesting that they have a role in pathogenicity in . The integrated expression profiles of the genes and metabolites involved in the glycolysis/gluconeogenesis and pentose phosphate pathways showed that the two major sugar metabolism-related pathways were completely changed, and GADP (glyceraldehyde-3-phosphate) may be a pivotal factor for CCR under different carbon sources. All these results provide a more comprehensive perspective for further analyzing the role of Cre in CCR.
碳分解代谢物阻遏（CCR）是微生物用来有效利用碳营养物质的一种常见转录调控机制，这对于微生物的适应性和病原种引起感染的能力至关重要。在这里，我们对导致棉花黄萎病的两个 CCR 基因 和 进行了特征描述。 和 敲除突变体表现出生长缓慢，分生孢子和微菌核产生减少，对棉花的毒力降低，表明 和 参与 的生长和致病性。我们进一步生成了 36 个高度可靠和稳定的 和 文库，以全面探索在不同碳源和 CCR 条件下生长时基因和代谢物的动态表达。基于加权基因共表达网络分析（WGCNA）和相关网络， 与许多下调基因共表达。这些基因网络跨越多个功能途径，其中包括七个基因，包括 PYCR（吡咯啉-5-羧酸盐还原酶），是 的潜在靶基因。不同的碳源条件触发了完全不同的基因调控网络，但它们在代谢途径中表现出相似的变化。包括 6-磷酸葡萄糖酸内酯酶和 2-ODGH（2-氧戊二酸脱氢酶 E1）在内的六个基因可能是该过程中的枢纽基因。 和 都可以全面影响植物细胞壁降解酶（PCWDE）基因的表达，表明它们在致病性中具有作用。参与糖酵解/糖异生和戊糖磷酸途径的基因和代谢物的综合表达谱表明，两个主要的糖代谢相关途径完全改变，并且 GADP（甘油醛-3-磷酸）可能是不同碳源下 CCR 的关键因素。所有这些结果为进一步分析 Cre 在 CCR 中的作用提供了更全面的视角。
