State Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai'an 271018, China.
Agronomy College, Shandong Agricultural University, Tai'an 271018, China.
Int J Mol Sci. 2024 Oct 30;25(21):11677. doi: 10.3390/ijms252111677.
Rye ( L.) genes, which contribute to the tertiary gene pool of wheat, include multiple disease resistance genes useful for the genetic improvement of wheat. Introgression lines are the most valuable materials for wheat breeding because of their small alien segments and limited or lack of linkage drag. In the present study, wheat-rye derivative lines SN21627-2 and SN21627-6 were produced via distant hybridization. A genomic in situ hybridization analysis revealed that SN21627-2 and SN21627-6 lack alien segments, while a multi-color fluorescence in situ hybridization analysis detected structural changes in both introgression lines. At the seedling and adult plant stages, SN21627-2 and SN21627-6 were highly resistant to stripe rust and powdery mildew. Primers for 86 PCR-based landmark unique gene markers and 345 rye-specific SLAF markers were used to amplify SN21627-2 and SN21627-6 genomic DNA. Eight markers specific to rye chromosome 2R were detected in both introgression lines, implying these lines carry chromosome 2R segments with genes conferring stripe rust and powdery mildew resistance. Therefore, SN21627-2 and SN21627-6 are resistant to more than one major wheat disease, making them promising bridging parents for breeding disease-resistant wheat lines.
黑麦（L.）基因是小麦三级基因库的组成部分，其中包含许多对小麦遗传改良有用的抗病基因。导入系因其含有较小的异源片段且有限或缺乏连锁累赘，是小麦育种最有价值的材料。本研究通过远缘杂交创制了小麦-黑麦衍生系 SN21627-2 和 SN21627-6。基因组原位杂交分析表明，SN21627-2 和 SN21627-6 缺乏异源片段，而多色荧光原位杂交分析检测到这两个导入系均存在结构变化。在幼苗和成株期，SN21627-2 和 SN21627-6 对条锈病和白粉病表现出高度抗性。使用 86 个基于 PCR 的标志性独特基因标记和 345 个黑麦特异性 SLAF 标记引物对 SN21627-2 和 SN21627-6 基因组 DNA 进行扩增。在两个导入系中均检测到与黑麦 2R 染色体特异性的 8 个标记，表明这些系携带赋予条锈病和白粉病抗性的 2R 染色体片段。因此，SN21627-2 和 SN21627-6 对多种主要小麦病害具有抗性，使它们成为培育抗病小麦品系的有前途的桥梁亲本。
