Center for Water and Environmental Sensors, Department for Integrated Sensor Systems, University for Continuing Education Krems, Dr.-Karl-Dorrek-Straße 30, 3500 Krems an der Donau, Austria.
Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria.
Int J Mol Sci. 2024 Oct 30;25(21):11682. doi: 10.3390/ijms252111682.
is a significant opportunistic pathogen highly prevalent in the environment, requiring early detection methods to prevent infections in vulnerable individuals. The most specific aptamer for , F23, has been used for the development of various assays and sensors for early diagnosis and monitoring. In this study, a novel F23-based electrochemical aptasensor was designed using disposal gold screen-printed electrodes (Au-SPEs) with high reproducibility. Methylene blue (MB) was used as an exogenous indicator, which significantly amplified the electrochemical signal and improved the sensitivity of detection. The aptasensor explored a limit of detection (LOD) of 8 CFU·mL and high selectivity for over other interfering bacteria. Furthermore, it showed potential to detect in tap water samples, offering a point-of-care tool for rapidly controlling the growth of this bacterium in various applications.
是一种重要的机会性病原体，在环境中高度流行，需要早期检测方法来预防易感人群的感染。最特异的 适体 F23 已被用于开发各种用于早期诊断和监测的检测方法和传感器。在这项研究中，设计了一种基于 F23 的新型电化学适体传感器，使用具有高重现性的废弃金丝网印刷电极 (Au-SPE)。亚甲基蓝 (MB) 被用作外源指示剂，显著放大了电化学信号并提高了检测灵敏度。该适体传感器的检测限 (LOD) 为 8 CFU·mL，对 具有高选择性，优于其他干扰细菌。此外，它显示出在自来水样品中检测 的潜力，为在各种应用中快速控制该细菌的生长提供了一种即时检测工具。
