Orthopaedics and Traumatology Department, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Roma, Italy.
Orthopaedics and Traumatology Department, Università Cattolica del Sacro Cuore, 00168 Roma, Italy.
Int J Mol Sci. 2024 Oct 31;25(21):11715. doi: 10.3390/ijms252111715.
Sarcomas are a heterogeneous group of malignancies with a high mortality rate. Detection of circulating tumor-derived material, such as circulating RNA in the peripheral blood of patients, has shown to be useful in diagnosis, prediction of prognosis and disease monitoring in several malignancies. This systematic review aims to probe the existing methods for detecting circulating tumor-derived RNAs from patients affected by sarcoma and their possible clinical application. A systematic review of the literature indexed in PubMed was performed. Each article had to analyze circulating RNA in human specimens obtained from liquid biopsies of patients affected by sarcoma. A total of 26 articles were included. We evaluated 1381 patients; 72% were affected by bone sarcoma and 28% by soft tissue sarcoma. By PCR-based methods, all the studies investigated circulating tumor RNA, mostly in the peripheral blood. Nearly half of the authors investigated the tumor expression and/or release of miRNA (42%). Several authors pointed out that circulating tumor-derived RNA has proven to have potential application in a clinical setting for sarcomas. To the best of our knowledge, this is the first review in the literature to attempt to put together data specifically on ctRNA in patients affected by sarcoma.
肉瘤是一组具有高死亡率的异质性恶性肿瘤。在几种恶性肿瘤中，检测患者外周血中的循环肿瘤源性物质，如循环 RNA，已被证明在诊断、预后预测和疾病监测方面具有一定的应用价值。本系统综述旨在探讨目前用于检测肉瘤患者循环肿瘤源性 RNA 的方法及其可能的临床应用。我们对 PubMed 索引的文献进行了系统综述。每篇文章都必须分析从肉瘤患者液体活检中获得的人类标本中的循环 RNA。共纳入 26 篇文章。我们评估了 1381 名患者；72%的患者患有骨肉瘤，28%的患者患有软组织肉瘤。通过基于 PCR 的方法，所有研究均检测了外周血中的循环肿瘤 RNA。近一半的作者研究了 miRNA 的肿瘤表达和/或释放（42%）。几位作者指出，循环肿瘤源性 RNA 已被证明在肉瘤的临床应用中具有一定的潜力。据我们所知，这是文献中首次尝试将肉瘤患者的 ctRNA 数据汇总在一起。
