Guangdong Provincial Key Laboratory of Applied Botany & South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
University of Chinese Academy of Sciences, Beijing 100039, China.
Int J Mol Sci. 2024 Oct 31;25(21):11725. doi: 10.3390/ijms252111725.
Calmodulins (CaMs) and calmodulin-like proteins (CMLs) belong to families of calcium-sensors that act as calcium ion (Ca) signal-decoding proteins and regulate downstream target proteins. As a tropical halophyte, shows great resistance to multiple abiotic stresses, including high salinity/alkalinity, extreme drought, heat, and intense sunlight. However, investigations of calcium ion signal transduction involved in the stress responses of are limited. The and gene families have been identified and characterized in many other plant species. Nevertheless, there is limited available information about these genes in . In this study, a bioinformatic analysis, including the gene structures, conserved protein domains, phylogenetic relationships, chromosome distribution, and gene synteny, was comprehensively performed to identify and characterize and . A spatio-temporal expression assay in different organs and environmental conditions was then conducted using the RNA sequencing technique. Additionally, several and members were then cloned and functionally characterized using the yeast heterogeneous expression system, and some of them were found to change the tolerance of yeast to heat, salt, alkalinity, and high osmotic stresses. The results of this study provide a foundation for understanding the possible roles of the and genes, especially for halophyte 's natural ecological adaptability for its native habitats. This study also provides a theoretical basis for further study of the physiological and biochemical functions of plant s and s that are involved in tolerance to multiple abiotic stresses.
钙调蛋白（CaMs）和钙调蛋白样蛋白（CMLs）属于钙传感器家族，作为钙离子（Ca）信号解码蛋白发挥作用，调节下游靶蛋白。作为一种热带盐生植物，表现出对多种非生物胁迫的高度抗性，包括高盐/碱性、极端干旱、高温和强烈阳光。然而，对钙离子信号转导在 胁迫反应中的作用的研究有限。在许多其他植物物种中已经鉴定和描述了 和 基因家族。然而，在 中，这些基因的信息有限。在这项研究中，进行了生物信息学分析，包括基因结构、保守蛋白结构域、系统发育关系、染色体分布和基因同线性，以鉴定和描述 和 。然后使用 RNA 测序技术在不同器官和环境条件下进行时空表达分析。此外，还使用酵母异源表达系统克隆和功能表征了几个 和 成员，发现其中一些成员改变了酵母对热、盐、碱和高渗胁迫的耐受性。本研究为理解 基因的可能作用提供了基础，特别是对盐生植物适应其原生栖息地的自然生态适应性。本研究还为进一步研究植物 和 参与耐受多种非生物胁迫的生理生化功能提供了理论依据。
