Institute of Mathematics, University of Gdansk, 80-308 Gdańsk, Poland.
iYoni App-For Fertility Treatment, LifeBite, 10-763 Olsztyn, Poland.
Int J Mol Sci. 2024 Nov 1;25(21):11749. doi: 10.3390/ijms252111749.
This study explores the proteomic composition of follicular fluid (FF) from women undergoing oocyte retrieval for in vitro fertilisation (IVF), with a focus on the effects of polycystic ovary syndrome (PCOS). FF samples were collected from 74 patients, including 34 with PCOS and 40 oocyte donors. Proteomic profiling using machine learning identified significant differences in protein abundance between the PCOS and control groups. Of the 484 quantified proteins, 20 showed significantly altered levels in the PCOS group. Functional annotation and pathway enrichment analysis pointed to the involvement of protease inhibitors and immune-related proteins in the pathophysiology of PCOS, suggesting that inflammation and immune dysregulation may play a key role. Additionally, HDL assembly was identified as a significant pathway, with apolipoprotein-AI (APOA1) and alpha-2-macroglobulin (A2M) as the major proteins involved. Notably, myosin light polypeptide 6 was the most downregulated protein, showing the highest absolute fold change, and may serve as a novel independent biomarker for PCOS.
这项研究探索了接受体外受精（IVF）取卵的女性卵泡液（FF）的蛋白质组组成，重点研究了多囊卵巢综合征（PCOS）的影响。从 74 名患者中收集了 FF 样本，包括 34 名 PCOS 患者和 40 名卵母细胞供体。使用机器学习进行蛋白质组分析，确定了 PCOS 组和对照组之间蛋白质丰度的显著差异。在定量的 484 种蛋白质中，有 20 种在 PCOS 组中表现出明显的改变。功能注释和途径富集分析表明，蛋白酶抑制剂和免疫相关蛋白参与了 PCOS 的病理生理学，表明炎症和免疫失调可能起关键作用。此外，还确定了 HDL 组装是一个重要途径，其中载脂蛋白-AI（APOA1）和α-2-巨球蛋白（A2M）是主要涉及的蛋白质。值得注意的是，肌球蛋白轻链 6 是下调最明显的蛋白质，表现出最高的绝对折叠变化，可能作为 PCOS 的一种新的独立生物标志物。
