Department of Plant Resources, Kongju National University, Yesan 32439, Republic of Korea.
Legumes Green Manure Resource Center, Kongju National University, Yesan 32439, Republic of Korea.
Int J Mol Sci. 2024 Nov 2;25(21):11784. doi: 10.3390/ijms252111784.
Laccase is an enzyme that plays an important role in fungi, including lignin degradation, stress defense, and formation of fruiting bodies. is a white-rot fungus in the Basidiomycota phylum, capable of delignifying wood. In this study, seven genes belonging to the laccase family were identified through de novo sequencing, containing Cu-Oxidase, Cu-Oxidase_2, and Cu-Oxidase_3 domains. Subsequently, the physical characteristics, phylogenetic relationships, protein secondary structure, and tertiary structure of the laccase family (-) were analyzed. Prediction of N-glycosylation sites identified 2 to 10 sites in the laccase family, with having the highest number of sites at 10. Sequence alignment and analysis of the laccase family showed high consistency in signature sequences. Phylogenetic analysis confirmed the relationship among laccases within the family, with - and - being closely positioned on the tree, exhibiting high similarity in tertiary structure predictions. This study identified and analyzed laccase family genes in using de novo sequencing, offering a simple method for identifying and analyzing the laccase family in organisms with unknown genetic information.
漆酶是一种在真菌中发挥重要作用的酶，包括木质素降解、应激防御和子实体形成。 是担子菌门中的一种白色腐朽真菌，能够木质素分解木材。在这项研究中，通过从头测序鉴定了属于漆酶家族的 7 个基因，包含 Cu-Oxidase、Cu-Oxidase_2 和 Cu-Oxidase_3 结构域。随后，分析了漆酶家族（-）的物理特性、系统发育关系、蛋白质二级结构和三级结构。对 N-糖基化位点的预测确定了漆酶家族中的 2 到 10 个位点，其中 具有 10 个位点的最高数量。漆酶家族的序列比对和分析显示了特征序列的高度一致性。系统发育分析证实了家族内漆酶之间的关系，-和-在树上紧密排列，在三级结构预测方面表现出高度相似性。本研究通过从头测序鉴定和分析了 中的漆酶家族基因，为鉴定和分析未知遗传信息的生物体中的漆酶家族提供了一种简单的方法。
