Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, China.
Institute of Advanced Study, Jiangxi University of Chinese Medicine, Nanchang 330004, China.
Int J Mol Sci. 2024 Nov 4;25(21):11834. doi: 10.3390/ijms252111834.
Fabricius (Cerambycidae: Lamiinae) is an important pest that damages kapok trees in Southeast Asia with a wide adaptability to temperature. Glycerol is a protectant and energy source for insects in low-temperature environments. However, glycerol biosynthesis-related genes at the molecular level are limited in . In this study, the supercooling points and freezing points at different stages were measured, and the cold hardiness of male and female pupae significantly differed. Moreover, a full-length transcriptome of was established; glycerol kinase (GK) and glycerol-3-phosphate dehydrogenase (GPDH) genes, which are related to glycerol metabolism, were identified, with a special focus on their expression profiles. A total of 24,476 isoforms stemmed from the full-length transcriptome, along with 568 lncRNAs, 56 transcription factor (TF) families, and 1467 alternative splicing (AS) events. The KEGG pathway enrichment analysis revealed that the isoforms associated with AS were enriched primarily in glycerolipid and glycerophospholipid metabolism. In total, three GK genes and one GPDH gene were identified, and GcGK1 and GcGK3 presented differential sex expression during the pupal stage, which may play a role in thermal adaptability. This study provides a valuable transcriptional database of and helps to elucidate the function of glycerol in the thermal adaptation mechanism of longhorn beetles.
鬼脸天牛（Cerambycidae：Lamiinae）是一种重要的害虫，它对东南亚的木棉树造成损害，对温度具有广泛的适应性。甘油是昆虫在低温环境下的保护剂和能量源。然而，在分子水平上，与甘油生物合成相关的基因在 中是有限的。在本研究中，测量了不同阶段的过冷点和冰点，并且雌雄蛹的抗寒性差异显著。此外，建立了 的全长转录组；鉴定了与甘油代谢相关的甘油激酶（GK）和甘油-3-磷酸脱氢酶（GPDH）基因，特别关注它们的表达谱。全长转录组共产生了 24476 个异构体，其中包括 568 个 lncRNA、56 个转录因子（TF）家族和 1467 个可变剪接（AS）事件。KEGG 途径富集分析表明，与 AS 相关的异构体主要富集在甘油脂质和甘油磷脂代谢中。总共鉴定出三个 GK 基因和一个 GPDH 基因，GcGK1 和 GcGK3 在蛹期表现出不同的性别表达，这可能在热适应性中发挥作用。本研究为 提供了有价值的转录数据库，并有助于阐明甘油在天牛热适应机制中的作用。
