KM Science Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 34054, Republic of Korea.
Institute of Traditional Medicine and Bioscience, Daejeon University, 62 Daehak-ro, Dong-gu, Daejeon 34520, Republic of Korea.
Nutrients. 2024 Oct 31;16(21):3732. doi: 10.3390/nu16213732.
Exposure to air pollution poses a risk to human respiratory health, and a preventive and therapeutic remedy against fine dust-induced respiratory disease is needed.
BACKGROUND/OBJECTIVES: The respiratory-protective effects of (LM) against airway inflammation were evaluated in a mouse model exposed to a fine dust mixture of diesel exhaust particles and particulate matter with a diameter of less than 10 µm (PM10D).
To induce airway inflammation, PM10D was intranasally injected into BALB/c mice three times a day for 12 days, and LM extracts were given orally once per day. The immune cell subtypes, histopathology, and expression of inflammatory mediators were analyzed from the bronchoalveolar lavage fluid (BALF) and lungs.
LM alleviated the accumulation of neutrophils and the number of inflammatory cells in the lungs and the BALF of the PM10D-exposed mice. LM also reduced the release of inflammatory mediators (MIP-2, IL-17, IL-1α, CXCL1, TNF-α, MUC5AC, and TRP receptor channels) in the BALF and lungs. Lung histopathology was used to examine airway inflammation and the accumulation of collagen fibers and inflammatory cells after PM10D exposure and showed that LM administration improved this inflammation. Furthermore, LM extract inhibited the MAPK and NF-κB signaling pathway in the lungs and improved expectoration activity through an increase in phenol red release from the trachea.
LM alleviated PM10D-exposed neutrophilic airway inflammation by suppressing MAPK/NF-κB activation. This study indicates that LM extract may be an effective therapeutic agent against inflammatory respiratory diseases.
评估（LM）对细粉尘混合物（柴油机排气颗粒和直径小于 10 µm 的颗粒物（PM10D））诱导的呼吸道疾病的呼吸保护作用。
为了诱导气道炎症，将 PM10D 每天三次鼻腔内注射到 BALB/c 小鼠中，共 12 天，每天口服一次 LM 提取物。从支气管肺泡灌洗液（BALF）和肺部分析免疫细胞亚型、组织病理学和炎症介质的表达。
LM 减轻了 PM10D 暴露小鼠肺部和 BALF 中中性粒细胞的积累和炎症细胞的数量。LM 还减少了 BALF 和肺部中炎症介质（MIP-2、IL-17、IL-1α、CXCL1、TNF-α、MUC5AC 和 TRP 受体通道）的释放。肺组织病理学用于检查气道炎症和 PM10D 暴露后胶原纤维和炎症细胞的积累，表明 LM 给药改善了这种炎症。此外，LM 提取物通过增加气管中苯酚红的释放来抑制肺部的 MAPK 和 NF-κB 信号通路，并通过增加痰液分泌来改善排痰活性。
LM 通过抑制 MAPK/NF-κB 激活减轻 PM10D 暴露引起的嗜中性气道炎症。本研究表明，LM 提取物可能是一种治疗炎症性呼吸道疾病的有效治疗剂。
