Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, 460 Iksandae-ro, Iksan 54538, Jeonbuk, Republic of Korea.
Nutrients. 2024 Oct 31;16(21):3737. doi: 10.3390/nu16213737.
BACKGROUND/OBJECTIVES: Alnustone (Aln) is an effective compound of Hayata. Aln possesses various pharmacological activities such as antibacterial, anti-inflammatory, and anti-cancer effects. However, the inhibitory effect of Aln on colorectal cancer (CRC) has not yet been identified. Thus, research was conducted to clarify whether Aln can suppress the proliferative and metastatic ability of CRC cells.
A cell viability assay was performed to confirm the decrease in CRC cell viability following Aln treatment. Flow cytometry was carried out to evaluate the effects of Aln on cell cycle arrest, autophagy, and apoptosis in CRC cells. In addition, a lung metastasis animal model was used to check the inhibitory effect of Aln on the metastasis of CRC cells.
Aln remarkably diminished the viability and colony-forming ability of several CRC cell lines. In addition, Aln led to a halt at the G0/G1 phase through downregulating cyclin D1-CDK4 in CRC cells. The upregulation of LC3B and p62 expression by Aln triggered autophagy of CRC cells. Moreover, Aln promoted mitochondrial depolarization, resulting in apoptosis of CRC cells. Oral administration of Aln significantly restrained the metastasized lung tumor nodules.
This study demonstrated that Aln can suppress the survival and lung metastasis of CRC cells by promoting cell cycle arrest, autophagy, and apoptosis.
背景/目的：岩白菜素（Aln）是岩白菜属的有效成分。Aln 具有多种药理活性，如抗菌、抗炎和抗癌作用。然而，Aln 对结直肠癌（CRC）的抑制作用尚未确定。因此，本研究旨在阐明 Aln 是否能抑制 CRC 细胞的增殖和转移能力。
通过细胞活力测定来证实 Aln 处理后 CRC 细胞活力的降低。通过流式细胞术评估 Aln 对 CRC 细胞周期阻滞、自噬和凋亡的影响。此外，还使用肺转移动物模型来检查 Aln 对 CRC 细胞转移的抑制作用。
Aln 显著降低了几种 CRC 细胞系的活力和集落形成能力。此外，Aln 通过下调 CRC 细胞中的细胞周期蛋白 D1-CDK4 导致细胞停滞在 G0/G1 期。Aln 上调 LC3B 和 p62 的表达引发 CRC 细胞的自噬。此外，Aln 促进线粒体去极化，导致 CRC 细胞凋亡。口服 Aln 显著抑制转移的肺肿瘤结节。
本研究表明，Aln 通过促进细胞周期阻滞、自噬和凋亡来抑制 CRC 细胞的存活和肺转移。
