L. 乙醇提取物：对Caco-2细胞系抗氧化活性和细胞毒性的体外评估。

L. Ethanolic Extracts: In Vitro Evaluation of Antioxidant Activity and Cytotoxicity on Caco-2 Cell Line.

作者信息

Pricop Maria-Alexandra, Lukinich-Gruia Alexandra Teodora, Cristea Iustina-Mirabela, Păunescu Virgil, Tatu Călin Adrian

机构信息

OncoGen Centre, County Hospital Pius Branzeu, 156 Liviu Rebreanu Blvd., 300736 Timisoara, Romania.

Department of Applied Chemistry and Environmental Engeneering and Inorganic Compounds, Faculty of Industrial Chemistry, Biotechnology and Environmental Engeneering, Polytechnic University of Timisoara, Vasile Pârvan 6, 300223 Timisoara, Romania.

出版信息

Plants (Basel). 2024 Oct 25;13(21):2987. doi: 10.3390/plants13212987.

DOI:10.3390/plants13212987

PMID:39519906

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11547917/

Abstract

sp. plants are used in traditional medicine because of their immunostimulatory and anticarcinogenic properties, despite their content of aristolochic acids (AAs), carcinogenic and nephrotoxic agents. Therefore, ethanolic extracts of leaves, a specie growing in Western Romania, were obtained to study antioxidant and cytotoxic effects. The antioxidant capacity of the extract was evaluated by five in vitro chemical-based assays, proving that ABTS assay was a better method for this type of evaluation showing an IC of 160.89 ± 0.21 µg/mL. Furthermore, the cytotoxic effects of the extract were established by an IC of 216 µg/mL for 24 h by MTT assay, followed by a cell-based assay on Caco-2 cells by the ABTS method. The antioxidant effects of the extract demonstrate potential therapeutic applications in complementary medicine.

摘要

尽管某些植物含有马兜铃酸（AAs）这种致癌和肾毒性物质，但由于其具有免疫刺激和抗癌特性，仍被用于传统医学。因此，我们获取了罗马尼亚西部生长的一种植物的叶子的乙醇提取物，以研究其抗氧化和细胞毒性作用。通过五种基于化学的体外试验评估了提取物的抗氧化能力，结果证明ABTS试验是进行此类评估的更好方法，其IC50为160.89±0.21μg/mL。此外，通过MTT试验确定提取物在24小时时的细胞毒性IC50为216μg/mL，随后通过ABTS方法在Caco-2细胞上进行基于细胞的试验。提取物的抗氧化作用表明其在补充医学中具有潜在的治疗应用。