Department of Pediatric Dentistry, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin, 300041, People's Republic of China.
Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin, 300041, People's Republic of China.
Int J Nanomedicine. 2024 Nov 6;19:11343-11356. doi: 10.2147/IJN.S475872. eCollection 2024.
The primary goals of endodontic therapy are to eliminate microbes and prevent reinfection. Persistent root canal infections and failure of root canal therapy are primarily attributed to the presence of bacteria, particularly E. faecalis. Chemical irrigants play a crucial role in complementing mechanical instrumentation in ensuring adequate disinfection. However, current techniques and available irrigants are limited in their ability to achieve optimal sterilization of the root canal system. In this study, we developed a novel material called La@PCDs by combining CQD-PVA and lanthanum for root canal irrigation.
A one-pot hydrothermal method was used to prepare composites of lanthanum and CQD-PVA (La@PCDs). Scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy and the particle size were employed to characterize La@PCDs. ROS generation was evaluated by measuring the fluorescence intensity emitted at 525 nm from 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA). In vitro experiments were conducted to assess the effectiveness of the nanoparticles in combating Enterococcus faecalis and eradicating in situ biofilm eradication in root canal. Furthermore, cytotoxicity assessments were carried out to demonstrate the safety of La@PCDs.
SEM and FTIR results showed that La@PCDs were successfully prepared and exhibiting a homogeneous size distribution and irregular morphology. ROS assessment demonstrated that La@PCDs have a synergistic effect, promoting the production of a large number of ROS. This effect only occurred under acidic PH conditions. The inherent acidity in the biofilm microenvironment can act as internal stimulus. In vitro experiments revealed superior antibacterial efficiency under acidic conditions without causing significant cytotoxicity compared to the commonly used NaClO irrigant. The biosafety of La@PCDs was confirmed.
Compared to existing materials, these nanoparticles exhibit favorable antibacterial and anti-biofilm properties, along with improved biocompatibility. These findings emphasize the potential of the integrated La@PCDs as a promising option for enhancing root canal irrigation and disinfection.
牙髓治疗的主要目标是消除微生物并防止再感染。持续性根管感染和根管治疗失败主要归因于细菌的存在，特别是粪肠球菌。化学冲洗剂在补充机械仪器操作以确保充分消毒方面发挥着至关重要的作用。然而，目前的技术和可用的冲洗剂在实现根管系统的最佳灭菌方面能力有限。在这项研究中，我们通过将 CQD-PVA 和镧结合开发了一种称为 La@PCDs 的新型材料，用于根管冲洗。
采用一锅水热法制备镧和 CQD-PVA（La@PCDs）的复合材料。通过扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）光谱和粒径对 La@PCDs 进行了表征。通过测量 2'，7'-二氯二氢荧光素二乙酸酯（DCFH-DA）发出的荧光强度来评估 ROS 的产生。进行了体外实验以评估纳米颗粒在对抗粪肠球菌和根除根管内原位生物膜方面的效果。此外，还进行了细胞毒性评估以证明 La@PCDs 的安全性。
SEM 和 FTIR 结果表明成功制备了 La@PCDs，且其表现出均匀的粒径分布和不规则的形态。ROS 评估表明 La@PCDs 具有协同作用，可促进大量 ROS 的产生。这种作用仅在酸性 PH 条件下发生。生物膜微环境中的固有酸度可充当内部刺激物。与常用的 NaClO 冲洗剂相比，在酸性条件下，La@PCDs 具有优越的抗菌效率，而不会引起明显的细胞毒性。证实了 La@PCDs 的生物安全性。
与现有材料相比，这些纳米颗粒表现出良好的抗菌和抗生物膜特性，同时具有改善的生物相容性。这些发现强调了整合 La@PCDs 作为增强根管冲洗和消毒的有前途选择的潜力。
