Brazilian Center for Research in Energy and Materials (CNPEM), OKNational Laboratory of Bioscience (LNBio), Campinas, Brazil.
Department of Manufacturing and Materials Engineering, School of Mechanical Engineering, Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil.
Commun Biol. 2024 Nov 11;7(1):1437. doi: 10.1038/s42003-024-07106-4.
3D skin models have been explored as an alternative method to the use of animals in research and development. Usually, human skin equivalents comprise only epidermis or epidermis/dermis layers. Herein, we leverage 3D bioprinting technology to fabricate a full-thickness human skin equivalent with hypodermis (HSEH). The collagen hydrogel-based structure provides a mimetic environment for skin cells to adhere, proliferate and differentiate. The effective incorporation of the hypodermis layer is evidenced by scanning electron microscopy, immunofluorescence, and hematoxylin and eosin staining. The transcriptome results underscore the pivotal role of the hypodermis in orchestrating the genetic expression of a multitude of genes vital for skin functionality, including hydration, development and differentiation. Accordingly, we evidence the paramount significance of full-thickness human skin equivalents with hypodermis layer to provide an accurate in vitro platform for disease modeling and toxicology studies.
3D 皮肤模型已被探索作为在研究和开发中替代动物使用的方法。通常，人类皮肤等效物仅包含表皮或表皮/真皮层。在此，我们利用 3D 生物打印技术来制造具有皮下组织（HSEH）的全厚度人皮肤等效物。基于胶原水凝胶的结构为皮肤细胞提供了一个类似的环境，使其能够附着、增殖和分化。扫描电子显微镜、免疫荧光和苏木精和伊红染色证实了皮下组织层的有效掺入。转录组结果强调了皮下组织在协调对皮肤功能至关重要的多种基因的遗传表达中的关键作用，包括水合、发育和分化。因此，我们证明了具有皮下组织层的全厚度人皮肤等效物的重要意义，为疾病建模和毒理学研究提供了一个准确的体外平台。
