Cluster of Excellence on Plant Sciences (CEPLAS), Institute for Plant Sciences, University of Cologne, Cologne, Germany.
Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany.
Elife. 2024 Nov 12;13:RP96266. doi: 10.7554/eLife.96266.
Programmed cell death occurring during plant development (dPCD) is a fundamental process integral for plant growth and reproduction. Here, we investigate the connection between developmentally controlled PCD and fungal accommodation in roots, focusing on the root cap-specific transcription factor ANAC033/SOMBRERO (SMB) and the senescence-associated nuclease BFN1. Mutations of both dPCD regulators increase colonization by the beneficial fungus , primarily in the differentiation zone. mutants additionally exhibit hypercolonization around the meristematic zone and a delay of -induced root-growth promotion. This demonstrates that root cap dPCD and rapid post-mortem clearance of cellular corpses represent a physical defense mechanism restricting microbial invasion of the root. Additionally, reporter lines and transcriptional analysis revealed that expression is downregulated during colonization in mature root epidermal cells, suggesting a transcriptional control mechanism that facilitates the accommodation of beneficial microbes in the roots.
在植物发育过程中发生的程序性细胞死亡（dPCD）是植物生长和繁殖所必需的基本过程。在这里，我们研究了发育调控的 PCD 与真菌在根中适应之间的联系，重点关注根冠特异性转录因子 ANAC033/SOMBRERO（SMB）和衰老相关核酶 BFN1。这两个 dPCD 调节剂的突变体增加了有益真菌的定殖，主要是在分化区。突变体还表现出在分生组织区周围的过度定殖和 - 诱导的根生长促进的延迟。这表明根冠的 dPCD 和细胞尸体的快速死后清除是一种物理防御机制，限制了微生物对根的入侵。此外，报告基因系和转录分析显示，在成熟的根表皮细胞中， 表达在 定殖过程中被下调，这表明了一种转录控制机制，促进了有益微生物在根中的适应。
