Department of Plastic Surgery, Tongren Hospital of Wuhan University (Wuhan Third Hospital), Wuhan, 430060, People's Republic of China.
Department of Medicine, Jianghan University, Wuhan, 430056, People's Republic of China.
Int J Nanomedicine. 2024 Nov 8;19:11499-11516. doi: 10.2147/IJN.S486640. eCollection 2024.
The photo-activated thermo/gas antimicrobial nanocomposite hydrogel, Gel/PDA@BNN6, is composed of the nitric oxide (NO) carrier N, N'-di-sec-butyl-N, N'-dinitroso-p-phenylenediamine (BNN6), photothermal (PTT) material polydopamine nanoparticles (PDA NPs), and methacrylate gelatin (GelMA). This hydrogel can release NO gas in a stable and controlled manner, generating a localized photothermal effect when exposed to near-infrared laser light. This dual action promotes the healing of full-thickness skin wounds that are infected.
Gel/PDA@BNN6 was developed, and both in vitro and in vivo experiments were carried out to evaluate its structure, physicochemical properties, antibacterial effects, effectiveness in promoting infected wound healing, and biocompatibility.
Gel/PDA@BNN6 was successfully synthesized, exhibiting a porous three-dimensional lattice structure and excellent mechanical properties. It demonstrated highly efficient photothermal conversion, controllable nitric oxide delivery, strong bactericidal effects, and minimal cytotoxicity in vitro. In vivo, Gel/PDA@BNN6, when used with NIR therapy, showed significant anti-inflammatory effects, promoted collagen deposition, and stimulated vascular neoangiogenesis, which accelerated wound closure. Additionally, it displayed superior biocompatibility.
Gel/PDA@BNN6 has shown an explicit curative effect for infected wound healing, suggesting it has a good chance of being an antimicrobial dressing in the future.
光激活的热/气体抗菌纳米复合水凝胶 Gel/PDA@BNN6 由一氧化氮（NO）载体 N，N'-二仲丁基-N，N'-二亚硝基对苯二胺（BNN6）、光热（PTT）材料聚多巴胺纳米粒子（PDA NPs）和甲基丙烯酰化明胶（GelMA）组成。该水凝胶可以稳定且受控地释放 NO 气体，在近红外激光照射下产生局部光热效应。这种双重作用促进了感染的全层皮肤伤口的愈合。
开发了 Gel/PDA@BNN6，并进行了体外和体内实验来评估其结构、物理化学性质、抗菌效果、促进感染性伤口愈合的效果以及生物相容性。
成功合成了 Gel/PDA@BNN6，其具有多孔三维晶格结构和优异的机械性能。它表现出高效的光热转换、可控的一氧化氮释放、强大的杀菌效果和体外极小的细胞毒性。在体内，Gel/PDA@BNN6 在近红外治疗的配合下，表现出显著的抗炎作用，促进胶原蛋白沉积，并刺激血管新生，从而加速伤口闭合。此外，它还表现出优异的生物相容性。
Gel/PDA@BNN6 对感染性伤口愈合显示出明确的疗效，表明它很有希望成为未来的抗菌敷料。
