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基于计算模型的非酶荧光葡萄糖传感分析方法的开发。

Computational Model-Assisted Development of a Nonenzymatic Fluorescent Glucose-Sensing Assay.

机构信息

Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.

Center for Remote Health Technologies and Systems, Texas A&M Engineering Experiment Station, College Station, Texas 77843, United States.

出版信息

ACS Sens. 2024 Nov 22;9(11):6218-6227. doi: 10.1021/acssensors.4c02117. Epub 2024 Nov 13.

DOI:10.1021/acssensors.4c02117
PMID:39536779
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11590106/
Abstract

Deep-red fluorescence was implemented in this fully injectable, nonenzymatic glucose biosensor design to allow for better light penetration through the skin, particularly for darker skin tones. In this work, a novel method was developed to synthesize Cy5.5 labeled mannose conjugates (Cy5.5-mannobiose, Cy5.5-mannotriose, and Cy5.5-mannotetraose) to act as the fluorescent competing ligand in a competitive binding assay with the protein Concanavalin A acting as the recognition molecule. Using fluorescence anisotropy (FA) data, a computational model was developed to determine optimal concentration ratios of the assay components to allow for sensitive glucose measurements within the physiological range. The model was experimentally validated by measuring the glucose response via FA of the three Cy5.5-labeled mannose conjugates synthesized with Cy5.5-mannotetraose demonstrating the most sensitive response to glucose across the physiological range. The developed method may be broadly applied to a vast range of commercially available fluorescent dyes and opens up opportunities for glucose measurements using nonenzymatic assays.

摘要

在这个完全可注射、非酶葡萄糖生物传感器设计中实现了深红色荧光，以允许更好地穿透皮肤的光，特别是对于较深的肤色。在这项工作中，开发了一种新的方法来合成 Cy5.5 标记的甘露糖缀合物（Cy5.5-甘露二糖、Cy5.5-甘露三糖和 Cy5.5-甘露四糖），作为与作为识别分子的蛋白伴刀豆球蛋白 A 进行竞争性结合测定的荧光竞争配体。使用荧光各向异性 (FA) 数据，开发了一个计算模型来确定测定组件的最佳浓度比，以允许在生理范围内进行灵敏的葡萄糖测量。该模型通过 FA 测量三种合成的 Cy5.5-甘露四糖的 Cy5.5 标记的甘露糖缀合物的葡萄糖响应来实验验证，该方法对葡萄糖在生理范围内的响应最敏感。开发的方法可以广泛应用于各种市售的荧光染料，并为使用非酶测定法进行葡萄糖测量开辟了机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/1d93f92ca372/se4c02117_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/46a8d459f0e2/se4c02117_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/6191eedf529c/se4c02117_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/7c52dd846e17/se4c02117_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/2c1581fa5eef/se4c02117_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/319cb5bc7d64/se4c02117_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/1d93f92ca372/se4c02117_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/46a8d459f0e2/se4c02117_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/6191eedf529c/se4c02117_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/7c52dd846e17/se4c02117_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/2c1581fa5eef/se4c02117_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/319cb5bc7d64/se4c02117_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb21/11590106/1d93f92ca372/se4c02117_0006.jpg

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