产黑素拟杆菌亚种的卟啉色素沉着
The porphyrin pigmentation of subspecies of Bacteroides melaninogenicus.
作者信息
Shah H N, Bonnett R, Mateen B, Williams R A
出版信息
Biochem J. 1979 Apr 15;180(1):45-50. doi: 10.1042/bj1800045.
Various subspecies of Bacteroides melaninogenicus differ in their pigmentation. Subsp. asaccharolyticus produces protohaem almost exclusively, subsp. intermedicus both protohaem and a smaller proportion of protoporphyrin, and subsp. melaninogenicus mainly protoporphyrin with a trace of protohaem. As a consequence young colonies can be differentiated by their red fluorescence in u.v. light (365nm): subsp. asaccharolyticus does not fluoresce, subsp. intermedicus shows a limited fluorescence, and subsp. melaninogenicus shows a bright fluorescence. The pigments were isolated as the dimethyl esters of protohaemin and of protoporphyrin and identified by electronic spectroscopy, mass spectrometry and comparisons by t.l.c. Incorporation of delta-aminolaevulinate into these pigments was not detected, nor was porphobilinogen formation observed. Subsp. melaninogenicus grown in the presence of [14C]protohaemin formed [14C]protoporphyrin. This appears to represent a novel biological demetallation.
产黑素拟杆菌的各个亚种在色素沉着方面存在差异。解糖亚种几乎只产生原血红素，中间亚种产生原血红素以及比例较小的原卟啉，而产黑素亚种主要产生原卟啉并含有微量原血红素。因此，年轻菌落可通过在紫外光（365nm）下的红色荧光进行区分：解糖亚种不发荧光，中间亚种显示有限的荧光，而产黑素亚种显示明亮的荧光。这些色素以原血红素和原卟啉的二甲酯形式分离出来，并通过电子光谱、质谱以及薄层层析比较进行鉴定。未检测到δ-氨基乙酰丙酸掺入这些色素中，也未观察到胆色素原的形成。在[14C]原血红素存在下生长的产黑素亚种形成了[14C]原卟啉。这似乎代表了一种新的生物脱金属作用。
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