HiChIP for Plant Tissues.

While most epigenomics studies are based on a linear view of genome organization, the necessity to take the three-dimensional chromatin folding into account to understand transcriptional regulation is now clearly recognized. In the past years, approaches combining proximity-based ligation with high-throughput sequencing have opened the way to study long/short-range chromatin interactions and, thus, to analyze 3D chromatin organization. Among them, HiChIP, a protein-based method to capture chromatin interactions, gave rise to the most comprehensive view of the chromatin contacts involving specific chromatin components in a given system. Here, we describe a detailed procedure to produce HiChIP libraries starting from plant tissues.