银离子和硅酸钠基团对纳米羟基磷灰石抗菌和抗真菌性能的影响。

Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okólna 2, Wrocław, 50-422, Poland.

Frantsevich Institute for Problems of Materials Science of the NAS of Ukraine, Pritsaka, 3, Kyiv, 03142, Ukraine.

Sci Rep. 2024 Nov 26;14(1):29339. doi: 10.1038/s41598-024-80303-7.

Hydroxyapatite is one of the most widely used materials in biomedical applications in reparative and regenerative medicine. Doping of nanosized hydroxyapatite improves its bioactive properties, and thus, the synthesis of different types of nanohydroxyapatite with antimicrobial activity is a perspective route of modern materials science. In this study, undoped hydroxyapatite (HAp), hydroxyapatite doped with silver (HAp with 0.1, 0.5 and 1 mol% Ag ions), and silicate-substituted hydroxyapatite doped with silver (Si-HAp with 0.1, 0.5 and 1 mol% Ag ions) nanoparticles (NPs) were synthesized by microwave-assisted hydrothermal technique and sintered at 450 °C. The structural properties and composition of obtained hydroxyapatite NPs were investigated using X-ray powder diffraction (XRPD), Fourier-transformed infrared spectroscopy (FT-IR), and Energy-dispersive X-ray spectroscopy (EDS). The morphology of synthesized nanosized powders was detected using the high-resolution transmission electron microscopy (HRTEM) technique. The results of XRPD for all synthesized nanosized powders confirmed the presence of hydroxyapatite crystal structure. The FT-IR spectra confirmed the presence of functional groups characteristic of the hydroxyapatite structure. The EDS analysis of obtained materials has shown the presence of Ca, P, O, Si, and Ag elements. Significant differences in size and morphology of the obtained particles were found using HRTEM. The particles have an elongated, rod-like shape with subtle differences. Moreover, HAp doped with 1 mol% Ag ions and Si-HAp doped with 1 mol% Ag ions nanosized powders showed antibacterial activity in comparison to pure hydroxyapatite both against gram-positive and gram-negative bacterial strains (Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis). These two types of hydroxyapatite NPs also demonstrated antifungal activity against reference strains of Candida albicans, Candida kruzei, and Candida tropicalis, with stronger activity observed for Si-HAp doped with silver.

羟基磷灰石是修复和再生医学中生物医学应用中最广泛使用的材料之一。纳米羟基磷灰石的掺杂可以改善其生物活性，因此，合成具有抗菌活性的不同类型的纳米羟基磷灰石是现代材料科学的一个有前途的途径。在这项研究中，通过微波辅助水热技术合成了未掺杂的羟基磷灰石（HAp）、掺杂 0.1、0.5 和 1 mol%Ag 离子的羟基磷灰石（HAp 与 0.1、0.5 和 1 mol%Ag 离子）、掺杂银的硅取代羟基磷灰石（Si-HAp）纳米粒子（NPs），并在 450°C 下烧结。使用 X 射线粉末衍射（XRPD）、傅里叶变换红外光谱（FT-IR）和能量色散 X 射线光谱（EDS）研究了所得羟基磷灰石 NPs 的结构性质和组成。使用高分辨率透射电子显微镜（HRTEM）技术检测了合成的纳米粉末的形貌。所有合成的纳米粉末的 XRPD 结果均证实了羟基磷灰石晶体结构的存在。FT-IR 谱证实了羟基磷灰石结构特征官能团的存在。EDS 分析表明，所得材料中存在 Ca、P、O、Si 和 Ag 元素。使用 HRTEM 发现，获得的粒子在尺寸和形态上有显著差异。这些粒子具有细长的棒状形状，略有不同。此外，与纯羟基磷灰石相比，掺杂 1 mol%Ag 离子的 HAp 和掺杂 1 mol%Ag 离子的 Si-HAp 纳米粉末对革兰氏阳性和革兰氏阴性菌（肺炎克雷伯菌、铜绿假单胞菌、表皮葡萄球菌、金黄色葡萄球菌、粪肠球菌）均表现出抗菌活性。这两种类型的羟基磷灰石 NPs 对白色念珠菌、克鲁兹假丝酵母和热带假丝酵母的参考菌株也表现出抗真菌活性，其中掺杂银的 Si-HAp 表现出更强的活性。