Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, 00100 Helsinki, Finland.
Faculty of Pharmaceutical Sciences, University of Bordeaux, 33076 Bordeaux, France.
Int J Mol Sci. 2024 Nov 7;25(22):11978. doi: 10.3390/ijms252211978.
species have been used in traditional medicine to treat fever and inflammation. However, there is no reported information on the antibacterial activities of and , and little is known about the phytochemistry of . In this study, winter-dormant twig extracts of , , and were screened for their antibacterial activities against , , , and . The antibacterial effects were evaluated using agar diffusion and turbidimetric microplate methods. Time-kill effects were measured using the microplate optical density (OD620) method. UPLC-PDA-QTOF/MS analysis was conducted to identify the polyphenols present in a methanol extract of . The antibacterial results show that methanol and hot and cold water twig extracts of , , and have significant antibacterial effects against , , and with the diameters of the inhibition zones (IZDs) ranging from 16.17 to 30.0 mm and the MICs between 1250 and 2500 µg/mL. Only the cold water extract of was moderately active against . Proanthocyanidins, procyanidin B1 (/ 577), and procyanidin C1 (/ 865) were identified as the major polyphenols present in the methanol extract of twigs for the first time. Additionally, salicin-7-sulfate was present in twigs. Procyanidin B-1, taxifolin, trans-p-hydroxycinnamic acid, and catechin showed growth inhibitory activity against with a MIC value of 250 µg/mL.
已经有一些物种被用于传统医学中，以治疗发热和炎症。然而，目前还没有关于 和 的抗菌活性的报道信息，并且对于 的植物化学也知之甚少。在这项研究中，我们对 、 、 和 的冬季休眠小枝提取物进行了筛选，以检测它们对 、 、 和 的抗菌活性。使用琼脂扩散和浊度微板法评估了抗菌效果。使用微板光密度（OD620）法测量了杀菌效果。通过 UPLC-PDA-QTOF/MS 分析鉴定了甲醇提取物中的多酚类物质。抗菌结果表明， 、 、 和 的甲醇和热水及冷水小枝提取物对 、 、 和 具有显著的抗菌作用，抑菌圈直径（IZD）范围为 16.17 至 30.0 毫米，MIC 值在 1250 至 2500 µg/mL 之间。只有 的冷水提取物对 有中度活性。原花青素、原花青素 B1（/577）和原花青素 C1（/865）首次被鉴定为 小枝甲醇提取物中的主要多酚类物质。此外，在 小枝中还存在水杨酸-7-硫酸盐。表儿茶素没食子酸酯、杨梅素、反式对羟基肉桂酸和儿茶素对 表现出抑制生长活性，MIC 值为 250 µg/mL。
