Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci. 2024 Nov 16;25(22):12301. doi: 10.3390/ijms252212301.
Phytopathogenic oomycetes, particularly , the causal agent of Phytophthora blight disease in essential vegetables and fruit crops, remains a persistent challenge in the vegetable production industry. However, the core molecular regulators of the pathophysiology and broad-range host characteristics of remain unknown. Here, we used transcriptomics and CRISPR-Cas9 technology to functionally characterize the contributions of a novel gene () coding for a hypothetical protein with a tubulin-binding cofactor C domain with a putative chloroplast-targeting peptide (cTP) to the pathophysiological development of . We observed significant upregulation in the expression of during pathogen-host interactions. However, the vegetative growth of the ∆ strains was not significantly different from the wild-type strains. gene replacement significantly compromised the sporulation, pathogenic differentiation, and virulence of . At the same time, ∆ strains were sensitive to cell wall stress-inducing osmolytes. These observations, coupled with the close evolutionary ties between and pathogenic oomycetes and algae, partly support the notion that is a conserved determinant of pathogenesis. This study provides insights into the significance of tubulin-binding cofactors in and underscores the potential of PcTbcc1 as a durable target for developing anti-oomycides to control phytopathogenic oomycetes.
植物病原卵菌，特别是引起重要蔬菜和水果作物疫霉病的病原菌，仍然是蔬菜生产行业面临的一个持续挑战。然而，其病理生理学和广泛宿主特性的核心分子调节因子仍不清楚。在这里，我们使用转录组学和 CRISPR-Cas9 技术，从功能上对一个新基因 ()进行了表征，该基因编码一个具有微管结合辅助因子 C 结构域和假定的质体靶向肽 (cTP)的假设蛋白，以了解其对病理生理学发展的贡献。我们观察到在病原菌-宿主相互作用过程中， 的表达显著上调。然而，Δ 株的营养生长与野生型株没有显著差异。基因替换显著削弱了 的孢子形成、致病性分化和毒力。同时，Δ 株对细胞壁应激诱导的渗透物敏感。这些观察结果，加上 与致病卵菌和藻类之间的密切进化关系，部分支持了这样一种观点，即 是致病的保守决定因素。本研究深入了解了微管结合辅助因子在 中的重要性，并强调了 PcTbcc1 作为开发抗卵菌剂来控制植物病原卵菌的持久靶标的潜力。
