• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于多重交叉置换扩增的侧向流生物传感器，用于快速灵敏检测 。

Multiple cross displacement amplification-based lateral flow biosensor for rapid and sensitive detection of .

机构信息

Department of Gastroenterology, Affiliated Children's Hospital, Capital Institute of Pediatrics, Beijing, China.

Experimental Research Center, Capital Institute of Pediatrics, Beijing, China.

出版信息

Front Cell Infect Microbiol. 2024 Nov 14;14:1396330. doi: 10.3389/fcimb.2024.1396330. eCollection 2024.

DOI:10.3389/fcimb.2024.1396330
PMID:39611101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11602460/
Abstract

(, HP), recognized globally as one of the most widespread bacteria, serves as primary etiological agent for numerous gastroduodenal diseases, highlighting the urgent need to develop rapid and sensitive diagnostic method for infection. Here, we devised a new diagnostic test that merged multiple cross displacement amplification (MCDA) with nanoparticle-based lateral flow biosensor (LFB), termed HP-MCDA-LFB, to facilitate the rapid and sensitive detection of . The whole detection workflow, which includes stages such as DNA template extraction (15 min), MCDA pre-amplification (40 min), and result readout (2 min), was efficiently completed within 1 h. After optimization, the HP-MCDA-LFB assay demonstrated remarkable sensitivity in detecting , with a detection threshold as low as 60 fg of genomic DNA (56 copies) per microliter. Furthermore, the HP-MCDA-LFB assay also achieved a perfect specificity rate of 100%, exhibiting no cross-reactivity with non- isolates. Particularly, the clinical feasibility of HP-MCDA-LFB assay was validated using 40 antral mucosa samples, among which 17 tested positive for , which was in complete agreement with the results obtained from the rapid urease test. In conclusion, the HP-MCDA-LFB method developed in this study is a rapid, sensitive, and specific method for diagnosing infection, indicating great potential for eradication therapy.

摘要

(, HP), 作为全球分布最广泛的细菌之一，被认为是许多胃十二指肠疾病的主要病因，因此需要开发一种快速、灵敏的 感染诊断方法。在这里，我们设计了一种新的诊断测试，将多重交叉置换扩增 (MCDA) 与基于纳米颗粒的侧向流动生物传感器 (LFB) 相结合，称为 HP-MCDA-LFB，以促进 的快速和灵敏检测。整个检测工作流程包括 DNA 模板提取（约 15 分钟）、MCDA 预扩增（约 40 分钟）和结果读取（约 2 分钟）等阶段，在 1 小时内高效完成。经过优化，HP-MCDA-LFB 检测法在检测 方面表现出了很高的灵敏度，检测下限低至 60 fg 基因组 DNA（~56 拷贝）/微升。此外，HP-MCDA-LFB 检测法还具有 100%的完美特异性，与非 分离株无交叉反应。特别是，该研究通过 40 个胃窦黏膜样本验证了 HP-MCDA-LFB 检测法的临床可行性，其中 17 个样本检测出 呈阳性，与快速尿素酶试验的结果完全一致。综上所述，本研究开发的 HP-MCDA-LFB 方法是一种快速、灵敏、特异的 感染诊断方法，为 根除治疗提供了很大的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/3320eab8fd2f/fcimb-14-1396330-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/41fbf13cead8/fcimb-14-1396330-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/8a47190b4d36/fcimb-14-1396330-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/28abebe52dd9/fcimb-14-1396330-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/8b1dcc3eff29/fcimb-14-1396330-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/bf3e44e59f05/fcimb-14-1396330-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/8286ea8bf1cf/fcimb-14-1396330-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/b62b1aea247e/fcimb-14-1396330-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/3320eab8fd2f/fcimb-14-1396330-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/41fbf13cead8/fcimb-14-1396330-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/8a47190b4d36/fcimb-14-1396330-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/28abebe52dd9/fcimb-14-1396330-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/8b1dcc3eff29/fcimb-14-1396330-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/bf3e44e59f05/fcimb-14-1396330-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/8286ea8bf1cf/fcimb-14-1396330-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/b62b1aea247e/fcimb-14-1396330-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a0/11602460/3320eab8fd2f/fcimb-14-1396330-g008.jpg

相似文献

1
Multiple cross displacement amplification-based lateral flow biosensor for rapid and sensitive detection of .基于多重交叉置换扩增的侧向流生物传感器，用于快速灵敏检测 。
Front Cell Infect Microbiol. 2024 Nov 14;14:1396330. doi: 10.3389/fcimb.2024.1396330. eCollection 2024.
2
Establishment and Application of a Multiple Cross Displacement Amplification Coupled With Nanoparticle-Based Lateral Flow Biosensor Assay for Detection of .建立和应用多重交叉置换扩增与基于纳米粒子的侧向流动生物传感器检测方法检测.
Front Cell Infect Microbiol. 2019 Sep 23;9:325. doi: 10.3389/fcimb.2019.00325. eCollection 2019.
3
Establishment and Application of Multiple Cross Displacement Amplification Coupled With Lateral Flow Biosensor (MCDA-LFB) for Visual and Rapid Detection of in Clinical Samples.建立并应用多重交叉置换扩增与侧向流生物传感器（MCDA-LFB），实现临床样本中 的可视化和快速检测。
Front Cell Infect Microbiol. 2019 Apr 16;9:102. doi: 10.3389/fcimb.2019.00102. eCollection 2019.
4
Nanoparticle-based biosensor integrated with multiple cross-displacement amplification for visual and rapid identification of hepatitis B virus and hepatitis C virus.基于纳米颗粒的生物传感器集成多重交叉置换扩增技术用于可视化快速鉴定乙型肝炎病毒和丙型肝炎病毒
Microbiol Spectr. 2025 May 6;13(5):e0173824. doi: 10.1128/spectrum.01738-24. Epub 2025 Apr 15.
5
Rapid detection of monkeypox virus by multiple cross displacement amplification combined with nanoparticle-based biosensor platform.通过多重交叉置换扩增结合基于纳米颗粒的生物传感器平台快速检测猴痘病毒
J Med Virol. 2023 Feb;95(2):e28479. doi: 10.1002/jmv.28479.
6
Rapid Detection of spp. and Elimination of Carryover Using Multiple Cross Displacement Amplification Coupled With Nanoparticles-Based Lateral Flow Biosensor.利用多重交叉置换扩增结合基于纳米颗粒的侧向流生物传感器快速检测 spp. 并消除携带污染。
Front Cell Infect Microbiol. 2019 Mar 28;9:78. doi: 10.3389/fcimb.2019.00078. eCollection 2019.
7
Two target genes based multiple cross displacement amplification combined with a lateral flow biosensor for the detection of Mycobacterium tuberculosis complex.基于两个靶基因的多重交叉置换扩增与侧向流生物传感器联合检测结核分枝杆菌复合群。
BMC Microbiol. 2021 Oct 4;21(1):267. doi: 10.1186/s12866-021-02328-6.
8
A Rapid Detection of Using Multiple Cross Displacement Amplification Linked With Nanoparticle-Based Lateral Flow Biosensor.利用多重交叉置换扩增联合基于纳米颗粒的侧向流生物传感器快速检测 。
Front Cell Infect Microbiol. 2021 Sep 22;11:721547. doi: 10.3389/fcimb.2021.721547. eCollection 2021.
9
Multiple Cross Displacement Amplification Coupled with Lateral Flow Biosensor (MCDA-LFB) for rapid detection of Legionella pneumophila.多重交叉置换扩增联合侧流生物传感器（MCDA-LFB）快速检测嗜肺军团菌。
BMC Microbiol. 2022 Jan 8;22(1):20. doi: 10.1186/s12866-021-02363-3.
10
Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis.建立和应用多重交叉置换扩增联合纳米粒子基生物传感器方法检测百日咳博德特氏菌。
BMC Microbiol. 2020 Aug 24;20(1):263. doi: 10.1186/s12866-020-01945-x.

引用本文的文献

1
Development of a real-time multiple cross displacement amplification assay for rapid and highly sensitive detection of Streptococcus pyogenes.开发一种用于快速、高灵敏检测化脓性链球菌的实时多重交叉置换扩增检测方法。
Eur J Clin Microbiol Infect Dis. 2025 Jun 10. doi: 10.1007/s10096-025-05186-6.