Baltzell J K, Berdanier C D
J Nutr. 1985 Jan;115(1):104-10. doi: 10.1093/jn/115.1.104.
The interacting effects of sucrose or starch with corn or coconut oil on the lipogenic responses of rats to starvation-refeeding was studied. Rats were either ad libitum-fed or starved for 48 h and refed for 48 h. Four different diets were used: 65% starch-5% corn oil, 65% starch-5% coconut oil, 65% sucrose-5% corn oil, 65% sucrose-5% coconut oil. Lipogenesis was assessed in two ways: glucose-6-phosphate dehydrogenase (G6PD) activity, malic enzyme (ME) activity and percent liver lipid (expt 1) and tritium (3HOH) incorporation into fatty acids (expt 2). Starved-refed rats had more liver lipid, greater enzyme activity and greater 3H incorporation into fatty acids than ad libitum-fed rats. Sucrose-fed rats had more lipogenic activity than starch-fed rats. Rats fed coconut oil were more lipogenic than rats fed corn oil. There were highly significant correlation coefficients between the enzyme activities (G6PD and ME) and the percent liver lipid and between the enzyme activities and 3H incorporation into fatty acid. Analysis of variance of these data revealed significant dietary effects on these lipogenic responses to starvation-refeeding. We conclude that both dietary carbohydrate and lipid play a significant role in the determination of the magnitude of the lipogenic response to starvation-refeeding.
研究了蔗糖或淀粉与玉米油或椰子油的相互作用对大鼠饥饿再投喂脂肪生成反应的影响。大鼠要么自由采食，要么饥饿48小时后再投喂48小时。使用了四种不同的饮食：65%淀粉-5%玉米油、65%淀粉-5%椰子油、65%蔗糖-5%玉米油、65%蔗糖-5%椰子油。通过两种方式评估脂肪生成：葡萄糖-6-磷酸脱氢酶（G6PD）活性、苹果酸酶（ME）活性和肝脏脂质百分比（实验1）以及脂肪酸中氚（3HOH）的掺入量（实验2）。与自由采食的大鼠相比，饥饿再投喂的大鼠肝脏脂质更多、酶活性更高，且脂肪酸中3H的掺入量更高。蔗糖喂养的大鼠比淀粉喂养的大鼠具有更高的脂肪生成活性。喂食椰子油的大鼠比喂食玉米油的大鼠脂肪生成能力更强。酶活性（G6PD和ME）与肝脏脂质百分比之间以及酶活性与脂肪酸中3H掺入量之间存在高度显著的相关系数。对这些数据的方差分析显示，饮食对这些饥饿再投喂的脂肪生成反应有显著影响。我们得出结论，饮食中的碳水化合物和脂质在决定饥饿再投喂脂肪生成反应的程度方面都起着重要作用。
