储存对尿毒症患者血清中离子钙测量的影响。

Effect of storage on measurement of ionized calcium in serum of uremic patients.

作者信息

Nikolakakis N I, De Francisco A M, Rodger R S, Gaiger E, Goodship T H, Ward M K

出版信息

Clin Chem. 1985 Feb;31(2):287-9.

Abstract

We studied, in 70 acidotic and non-acidotic uremic patients, the analytical variance in serum ionized calcium as related to duration and temperature of storage. Storage of serum or whole blood at 4 degrees C for as long as 6 h did not significantly alter the measured concentration of ionized calcium in the serum. Storage at room temperature for 6 h, or longer at 4 degrees C or -20 degrees C, resulted in inaccuracies in 39 to 79% of the samples of serum and in 38 to 92% of the samples of whole blood. These errors were not negated by correcting the values for ionized calcium to a pH of 7.40. Indeed, corrected values for calcium were even more unreliable in acidotic patients. We conclude that samples from uremic patients should be analyzed for ionized calcium within 2 h, or within 6 h if stored at 4 degrees C.

摘要

我们在70例酸中毒和非酸中毒的尿毒症患者中，研究了血清离子钙的分析方差与储存时间和温度的关系。血清或全血在4℃下储存长达6小时，并未显著改变血清中离子钙的测量浓度。在室温下储存6小时，或在4℃或-20℃下储存更长时间，导致39%至79%的血清样本和38%至92%的全血样本出现误差。通过将离子钙的值校正到pH 7.40，这些误差并未消除。实际上，酸中毒患者中校正后的钙值更不可靠。我们得出结论，尿毒症患者的样本应在2小时内分析离子钙，如果在4℃下储存则应在6小时内分析。