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阿尔及利亚水果脱脂、未脱脂及油提取物的脂肪酸组成和抗氧化能力

Fatty acid composition and antioxidant capacity of defatted, non-defatted and oils extracts of fruit from Algeria.

作者信息

Guenane Hadjira, Bakchiche Boulanouar, Erenler Ramazan, Yildiz Ilyas, Khalaf Omar M, Saad Amal M, Ghareeb Mosad A

机构信息

Laboratory of Biological and Agricultural Sciences (LBAS), Amar Telidji University, Laghouat 03000, Algeria.

Department of Chemistry, Faculty of Arts and Sciences, Tokat, Gaziosmanpasa University, 60240 Tokat, Turkey.

出版信息

Rocz Panstw Zakl Hig. 2024 Dec 23;75(3):237-245. doi: 10.32394/rpzh/192523. Epub 2024 Sep 13.

Abstract

BACKGROUND

The nutritional value and health-promoting properties cause the fruits (acorns) of to have great potential for use in the food industry as functional ingredients and antioxidants source.

OBJECTIVE

In this study, the amount of total phenolic compounds, flavonoids in different extracts (defatted, non-defatted) and composition of fatty acids in the fruits oils of were investigated. Besides, antioxidant activity was determined.

MATERIAL AND METHODS

Fatty acids were extracted with n-hexane and determined by gas chromatography with mass spectrometry detection (GC-MS). Total phenolic and flavonoids contents in the extracts were measured spectrophotometrically and the antioxidant activities were tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl), free radical scavenging assay, free radical-scavenging ABTS and total antioxidant capacity.

RESULTS

The amount of total phenolic and flavonoid compounds in the defatted were 634.36±27.41 mg GAE/g DW and 96.85±2.13 mg RE/g DW, respectively. Unsaturated fatty acids were detected in higher amounts than saturated fatty acids. The primary unsaturated fatty acids of the oil were oleic acid (65.38%), 9,12-octadecadienoic acid (16.64%) and palmitic acid (12.81%). Besides, defatted extract showed remarkable DPPH and ABTS radical scavenging activity with IC values of 0.008±0.0008, 0.005±0.001 mg/ml respectively, while high total antioxidant capacity of the non-defatted extract with VCEAC value 0.13±0.006.

CONCLUSIONS

oil contained high amounts of polyphenols, high essential fatty acids and antioxidant potential for producing specific health promoting antioxidants in food and pharmaceutical industry.

摘要

背景

的果实(橡子)因其营养价值和促进健康的特性,在食品工业中作为功能性成分和抗氧化剂来源具有巨大的应用潜力。

目的

本研究调查了不同提取物(脱脂、未脱脂)中总酚类化合物、黄酮类化合物的含量以及果实油中脂肪酸的组成。此外,还测定了抗氧化活性。

材料与方法

用正己烷提取脂肪酸,采用气相色谱 - 质谱检测法(GC - MS)进行测定。用分光光度法测定提取物中总酚和黄酮类化合物的含量,并通过DPPH(2,2 - 二苯基 - 1 - 苦基肼)自由基清除试验、ABTS自由基清除试验和总抗氧化能力测试抗氧化活性。

结果

脱脂提取物中总酚类化合物和黄酮类化合物的含量分别为634.36±27.41 mg GAE/g DW和96.85±2.13 mg RE/g DW。检测到不饱和脂肪酸的含量高于饱和脂肪酸。油中的主要不饱和脂肪酸为油酸(65.38%)、9,12 - 十八碳二烯酸(16.64%)和棕榈酸(12.81%)。此外,脱脂提取物表现出显著的DPPH和ABTS自由基清除活性,IC值分别为0.008±0.0008、0.005±0.001 mg/ml,而未脱脂提取物具有较高的总抗氧化能力,VCEAC值为0.13±0.006。

结论

油含有大量的多酚、高含量的必需脂肪酸以及在食品和制药工业中生产特定的促进健康的抗氧化剂的潜力。

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