一种具有红色环丙基三嗪硫醚（Red-CPTS）的红光可激活内源性基因转录系统。
A Red Light-Activatable Endogenous Gene Transcription System with Red-CPTS.
作者信息
Nakajima Takahiro, Kuwasaki Yuto, Yamamoto Shota, Otabe Takahiro, Sato Moritoshi
机构信息
Kanagawa Institute of Industrial Science and Technology, Kawasaki-shi, Kanagawa, Japan.
Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
出版信息
Methods Mol Biol. 2025;2840:45-55. doi: 10.1007/978-1-0716-4047-0_4.
Red light penetrates deep into mammalian tissues and has low phototoxicity. We developed a red light-activatable photoswitch (MagRed) for deep tissue optogenetics. Using MagRed, we developed a red light-activatable endogenous gene transcription system (Red-CPTS) based on CRISPR-Cas9. Here we provide a detailed protocol for endogenous gene activation using Red-CPTS in cultured mammalian cells and living mice in vivo.
红光可深入穿透哺乳动物组织且光毒性较低。我们开发了一种用于深部组织光遗传学的红光可激活光开关（MagRed）。利用MagRed，我们基于CRISPR-Cas9开发了一种红光可激活的内源基因转录系统（Red-CPTS）。在此，我们提供了一份详细方案，介绍如何在培养的哺乳动物细胞和活体小鼠体内使用Red-CPTS进行内源基因激活。
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