Cantons Josep Maria, Bachhuka Akash, Marsal Lluis F
Department of Electronics, Electric, and Automatic Engineering, Rovira I Virgili University (URV), 43007 Tarragona, Spain.
Institute of Chemical Research of Catalonia (ICIQ), 43007 Tarragona, Spain.
Biosensors (Basel). 2024 Dec 13;14(12):610. doi: 10.3390/bios14120610.
Preeclampsia is a pregnancy-specific hypertensive syndrome recognized as the leading cause of maternal and fetal morbidity worldwide. Early diagnosis is crucial for mitigating its adverse effects, and recent investigations have identified endoglin as a potential biomarker for this purpose. Here, we present the development of a hybrid biosensor platform for the ultrasensitive detection of endoglin, aimed at enabling the early diagnosis of preeclampsia. This platform integrates the high surface area properties of nanoporous anodic alumina (NAA) with the unique optical characteristics of gold nanoclusters (AuNCs) to achieve enhanced detection capabilities. The NAA surface functionalized to promote attachment of AuNCs, which then was functionalized with specific antibodies to confer selectivity towards endoglin. Photoluminescence (PL) analysis of the biosensor demonstrated a linear detection range of 10-50 ng/mL, with a detection limit of 5.4 ng/mL and a sensitivity of 0.004 a.u./(ng/mL). This proof-of-concept study suggests that the NAA-AuNCs-based biosensing platform holds significant potential for the development of ultrasensitive, portable, and cost-effective diagnostic tools for preeclampsia, offering a promising avenue for advancing prenatal care.
子痫前期是一种特定于妊娠的高血压综合征，被认为是全球孕产妇和胎儿发病的主要原因。早期诊断对于减轻其不良影响至关重要，最近的研究已将内皮糖蛋白确定为用于此目的的潜在生物标志物。在此，我们展示了一种用于超灵敏检测内皮糖蛋白的混合生物传感器平台的开发，旨在实现子痫前期的早期诊断。该平台将纳米多孔阳极氧化铝（NAA）的高表面积特性与金纳米团簇（AuNCs）的独特光学特性相结合，以实现增强的检测能力。对NAA表面进行功能化处理以促进AuNCs的附着，然后用特异性抗体进行功能化处理，以赋予对内皮糖蛋白的选择性。生物传感器的光致发光（PL）分析显示线性检测范围为10 - 50 ng/mL，检测限为5.4 ng/mL，灵敏度为0.004 a.u./(ng/mL)。这项概念验证研究表明，基于NAA - AuNCs的生物传感平台在开发用于子痫前期的超灵敏、便携式和经济高效的诊断工具方面具有巨大潜力，为推进产前护理提供了一条有前景的途径。
