Ma Huazhong, Zou Yujun, Liu Liang, Yu Juan, Wang Peng, Lin Lin, Chen Meijuan, Jia Ruoxian, Fan Yimin
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Longpan Road 159, Nanjing 210037, China.
State Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China.
Int J Biol Macromol. 2025 Mar;297:139505. doi: 10.1016/j.ijbiomac.2025.139505. Epub 2025 Jan 7.
The weak cohesive strength of tissue adhesives hinders their practical applications. To overcome this challenge, we develop a green bio-adhesive that balances both cohesion and adhesion, drawing inspiration from the natural adhesion mechanisms of mussels. This bio-adhesive, referred to as OTS, was ingeniously crafted through the co-assembly of multi-surface-charged chitin nanofibers (OAChN) and tannic acid (TA), integrated with silk fibroin (SF), resulting in a material with enhanced cohesive strength and robust adhesive properties. The adhesive achieved significant cohesion through hydrogen-bonded crosslinking among OAChN, SF, and TA, boasting a tensile strength of 24.53 KPa and allowing for 150 % elastic deformation. OTS adheres effectively to diverse complex surfaces, with adhesive strengths of 14.55 MPa underwater and 8.83 MPa in air, demonstrating excellent versatility. The biocompatibility and degradability of OTS were confirmed by a wound healing model in SD rats, showing no nanotoxicity and effectively promoting wound healing, rapid hemostasis, and sealing. This green adhesive strategy offers a novel approach to augmenting the cohesive strength of tissue adhesives suitable for complex conditions and has potential medical applications ranging from rapid hemostasis to wound healing enhancement.
组织粘合剂较弱的内聚强度阻碍了它们的实际应用。为了克服这一挑战，我们从贻贝的天然粘附机制中汲取灵感，开发了一种兼具内聚力和粘附力的绿色生物粘合剂。这种生物粘合剂称为OTS，是通过多表面电荷的几丁质纳米纤维（OAChN）和单宁酸（TA）与丝素蛋白（SF）共同组装巧妙制成的，从而得到一种具有增强内聚强度和强大粘附性能的材料。该粘合剂通过OAChN、SF和TA之间的氢键交联实现了显著的内聚力，其拉伸强度为24.53千帕，可实现150%的弹性变形。OTS能有效粘附于各种复杂表面，在水下的粘附强度为14.55兆帕，在空气中为8.83兆帕，显示出优异的通用性。OTS的生物相容性和可降解性通过SD大鼠的伤口愈合模型得到证实，表明其无纳米毒性，并能有效促进伤口愈合、快速止血和封闭。这种绿色粘合剂策略为增强适用于复杂情况的组织粘合剂的内聚强度提供了一种新方法，并且在从快速止血到增强伤口愈合等方面具有潜在的医学应用。
