Dyrma Sherina, Pei Tong-Tong, Liang Xiaoye, Dong Tao
Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, Guangdong, China.
J Bacteriol. 2025 Mar 20;207(3):e0045524. doi: 10.1128/jb.00455-24. Epub 2025 Feb 4.
Protein secretion systems are critical macromolecular machines employed by bacteria to interact with diverse environments and hosts during their life cycle. Cytosolically produced protein effectors are translocated across at least one membrane to the outside of the cells or directly into target cells. In most secretion systems, these effectors are mere passengers in unfolded or folded states. However, the type VI secretion system (T6SS) stands out as a powerful contractile device that requires some of its effectors as structural components. This review aims to provide an updated view of the diverse functions of effectors, especially focusing on their roles in T6SS assembly, the implications for T6SS engineering, and the potential of recently developed T6SS models to study effector-T6SS association.
蛋白质分泌系统是细菌在其生命周期中用于与各种环境和宿主相互作用的关键大分子机器。在细胞质中产生的蛋白质效应物会穿过至少一层膜转运到细胞外或直接进入靶细胞。在大多数分泌系统中，这些效应物只是以未折叠或折叠状态的 “乘客”。然而，VI型分泌系统（T6SS）作为一种强大的收缩装置脱颖而出，它需要一些效应物作为结构成分。本综述旨在提供效应物多种功能的最新观点，特别关注它们在T6SS组装中的作用、对T6SS工程的影响以及最近开发的T6SS模型在研究效应物与T6SS关联方面的潜力。
