通过基于激发态分子内质子转移的近红外荧光探针监测中风期间细胞膜谷胱甘肽的动态变化
Monitoring Dynamic Changes of Cellular Membrane GSH During Stroke via an ESIPT-Based Near-Infrared Fluorescent Probe.
作者信息
Gao Yue, Wang Zhao
机构信息
Hubei Provincial Engineering Research Center of Racing Horse Detection and Application Transformation, Equine Science Research and Horse Doping Control Laboratory, College of Food Science and Technology, Wuhan Business University, Wuhan 430056, China.
出版信息
Molecules. 2025 Jan 28;30(3):592. doi: 10.3390/molecules30030592.
Stroke, primarily ischemic (85%), results from inadequate blood supply and is worsened by ferroptosis, characterized by free radical generation and lipid peroxidation. Monitoring ferroptosis is essential for understanding its mechanisms and developing treatments. Glutathione (GSH) is a key ferroptosis biomarker, but current probes are limited by short excitation/emission wavelengths, small Stokes shifts, and inability to monitor dynamic GSH changes at the cellular membrane, where ferroptosis plays a crucial role. To address these issues, we developed the PM-Red-GSH, a novel near-infrared (NIR) probe based on the Excited-state intramolecular proton transfer (ESIPT) mechanism. It shows strong NIR emission (715 nm), large Stokes shift (290 nm), and enhanced membrane binding (PCC = 0.95) due to its alkyl group. PM-Red-GSH enables dynamic GSH monitoring in an MCAO mouse model. These findings offer new insights into ferroptosis and stroke treatment.
中风主要是缺血性的（85%），由血液供应不足引起，并因铁死亡而恶化，其特征是自由基生成和脂质过氧化。监测铁死亡对于理解其机制和开发治疗方法至关重要。谷胱甘肽（GSH）是一种关键的铁死亡生物标志物，但目前的探针受到激发/发射波长短、斯托克斯位移小以及无法监测细胞膜上GSH动态变化的限制，而铁死亡在细胞膜上起着关键作用。为了解决这些问题，我们开发了PM-Red-GSH，这是一种基于激发态分子内质子转移（ESIPT）机制的新型近红外（NIR）探针。由于其烷基，它显示出强烈的近红外发射（715nm）、大的斯托克斯位移（290nm）和增强的膜结合（PCC = 0.95）。PM-Red-GSH能够在大脑中动脉闭塞（MCAO）小鼠模型中动态监测GSH。这些发现为铁死亡和中风治疗提供了新的见解。
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