SHORT INTERNODE1在水稻花器官发育过程中调控MADS转录因子的活性。

SHORT INTERNODE1 regulates the activity of MADS transcription factors during rice floral organ development.

作者信息

Duan Erchao, Teng Xuan, Xu Huan, Ma Wenyu, Zhang Desheng, Zhang Rushuang, Gu Chuanwei, Zhang Yipeng, Chen Rongbo, Chen Xiaoli, Feng Miao, Lin Qibing, Dong Hui, Zhang Yuanyan, Yang Xue, Zhou Lei, Liu Shijia, Liu Xi, Tian Yunlu, Jiang Ling, Wang Haiyang, Wang Yihua, Wan Jianmin

机构信息

State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Jiangsu Nanjing Rice Germplasm Resources National Field Observation and Research Station, Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing 210095, China.

State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

出版信息

Plant Physiol. 2025 Feb 7;197(2). doi: 10.1093/plphys/kiaf034.

DOI:10.1093/plphys/kiaf034

PMID:39964839

Abstract

Floral organ identity is fundamental to species diversity and reproductive success in plants and is mainly determined by the combinatorial action of MADS homeotic factors. However, despite their conserved roles in specifying floral organ identity, the regulation of MADS transcription factors remains elusive. Here, we show that the rice (Oryza sativa L.) short internode1 (shi1) mutant displays pleiotropic defects in floral organ development, resulting in severe penalties to yield and grain quality. OsSHI1 mRNA accumulates in each floral organ whorl, and OsSHI1 interacts with multiple MADS transcription factors, especially the Class E members. This physical interaction occurs through the intrinsic MADS domains, thus regulating the transcriptional activity of the MADS transcription factors. This study provides insight into the molecular and genetic regulatory mechanisms underlying the roles of OsSHI1 and MADS transcription factors in rice floral organ development and, consequently, grain yield and quality.

摘要

花器官特性对于植物的物种多样性和繁殖成功至关重要，并且主要由MADS同源异型因子的组合作用决定。然而，尽管它们在指定花器官特性方面具有保守作用，但MADS转录因子的调控仍不清楚。在这里，我们表明水稻（Oryza sativa L.）短节间1（shi1）突变体在花器官发育中表现出多效性缺陷，导致产量和籽粒品质严重下降。OsSHI1 mRNA在每个花器官轮中积累，并且OsSHI1与多个MADS转录因子相互作用，尤其是E类成员。这种物理相互作用通过内在的MADS结构域发生，从而调节MADS转录因子的转录活性。本研究深入了解了OsSHI1和MADS转录因子在水稻花器官发育以及产量和品质方面作用的分子和遗传调控机制。