Fu Rongtao, Zhao Liyu, Chen Cheng, Wang Jian, Chen Yu, Lu Daihua
Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066, China.
Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture and Rural Affairs, Chengdu 610066, China.
Biomolecules. 2025 Jan 28;15(2):186. doi: 10.3390/biom15020186.
Rice false smut (RFS), an emerging disease caused by the fungus (Cooke), reduces rice grain yield and quality in rice-planting regions worldwide. The identification of the genes or quantitative trait loci (QTLs) associated with RFS resistance is vital to resistance breeding and the mitigation of RFS damage. In this study, RFS resistance QTLs were located in the resistant variety IR77298-14-1-2::IRGC117374-1. A total of 4 RFS resistance QTLs were detected on rice chromosomes 1, 3, 5, and 12 in the F and F mapping populations using 119 polymorphic simple sequence repeat (SSR) molecular markers. Of these QTLs, and were repeatedly detected in both populations. Interestingly, QTL on chromosome 3 is a novel resistance locus that exhibited the largest phenotypic effect. These results suggest that SSR markers linked to are valuable for marker-assisted breeding for RFS resistance in rice. The prediction of putative candidate genes within revealed three resistance-related proteins containing an F-box domain, Myb-like DNA-binding domain, and kinase protein. In summary, our findings provide new QTLs/genes for resistance to RFS and will promote rice disease resistance through molecular-marker-assisted breeding.
稻曲病（RFS）是由真菌（库克）引起的一种新出现的病害，它降低了全球水稻种植区的稻谷产量和品质。鉴定与稻曲病抗性相关的基因或数量性状位点（QTL）对于抗性育种和减轻稻曲病危害至关重要。在本研究中，稻曲病抗性QTL定位在抗性品种IR77298-14-1-2::IRGC117374-1中。使用119个多态性简单序列重复（SSR）分子标记，在F和F作图群体的水稻第1、3、5和12号染色体上共检测到4个稻曲病抗性QTL。在这些QTL中，和在两个群体中均被重复检测到。有趣的是，第3号染色体上的QTL是一个新的抗性位点，表现出最大的表型效应。这些结果表明，与连锁的SSR标记对于水稻稻曲病抗性的标记辅助育种具有重要价值。对范围内推定候选基因的预测揭示了三种含有F-box结构域、Myb样DNA结合结构域和激酶蛋白的抗性相关蛋白。总之，我们的研究结果为稻曲病抗性提供了新的QTL/基因，并将通过分子标记辅助育种促进水稻抗病性。
