AlMobarak Sultanah, AlMadi Ebtissam, Almohaimede Amal, Badran Mohamed, Lambarte Rhodanne A
Department of Restorative Dental Sciences, Endodontic Division, College of Dentistry, King Saud University, Riyadh 11432, Saudi Arabia.
King Abdullah Bin Abdulaziz University Hospital, Princess Nourah University, Riyadh 13412, Saudi Arabia.
Int J Mol Sci. 2025 Feb 7;26(4):1412. doi: 10.3390/ijms26041412.
This study aimed to evaluate the effect of a newly formulated (CM) nano-irrigant on the morphology, viability, proliferation, migration, and wound healing of human bone marrow-derived mesenchymal stem cells (hBMMSCs). Different concentrations of CM nano-irrigant were prepared. The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined to be 25 and 30 mg/mL, respectively. The solution was dispersed into liposomes, which were subsequently coated with chitosan-forming chitosomes. Three concentrations of CM chitosomes were evaluated (25, 30, and 35 mg/mL) along with positive (5.25% NaOCl) and negative (basal culture media) control groups. Cellular viability and proliferation were quantified using AlamarBlue, while wound-healing ability was determined using the scratch assay, and 3D cellular migration was evaluated using the transwell migration assay. All tested concentrations induced observable changes in cellular morphology without any detrimental effects. Viability was monitored at 1, 6, and 24 h, with only Group 1 (25 mg/mL) showing no significant effect on cellular viability. Cellular proliferation was observed over 14 days, with Group 3 (35 mg/mL) being the only group that showed a significantly slower proliferative rate. All tested concentrations resulted in significant differences in transwell migration compared to the negative control. Significant differences were observed within each group across different time points (24-48 h). The results confirm the biocompatibility of the newly formulated CM nano-irrigant in terms of hBMMSCs' viability, proliferation, morphology, migration, and wound healing.
本研究旨在评估一种新配制的（CM）纳米冲洗剂对人骨髓间充质干细胞（hBMMSCs）的形态、活力、增殖、迁移和伤口愈合的影响。制备了不同浓度的CM纳米冲洗剂。确定其最低抑菌浓度（MIC）和最低杀菌浓度（MBC）分别为25和30mg/mL。将该溶液分散到脂质体中，随后用壳聚糖包被形成壳聚糖体。评估了三种浓度的CM壳聚糖体（25、30和35mg/mL）以及阳性（5.25%次氯酸钠）和阴性（基础培养基）对照组。使用AlamarBlue定量细胞活力和增殖，使用划痕试验测定伤口愈合能力，使用Transwell迁移试验评估三维细胞迁移。所有测试浓度均引起细胞形态的可观察变化，且无任何有害影响。在1、6和24小时监测活力，只有第1组（25mg/mL）对细胞活力无显著影响。在14天内观察细胞增殖，第3组（35mg/mL）是唯一显示增殖速率显著较慢的组。与阴性对照相比，所有测试浓度在Transwell迁移方面均有显著差异。在不同时间点（24 - 48小时）的每组内均观察到显著差异。结果证实了新配制的CM纳米冲洗剂在hBMMSCs的活力、增殖、形态、迁移和伤口愈合方面的生物相容性。
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