De Swastik, Zhou Michelle, Brown Zuben P, Burton-Smith Raymond N, Hashem Yaser, Pestova Tatyana V, Hellen Christopher U T, Frank Joachim
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA.
Irvington High School, Irvington, New York 10533, USA.
RNA. 2025 May 16;31(6):781-790. doi: 10.1261/rna.080294.124.
Examination of all publicly available (rabbit) ribosome cryo-EM structures reveals numerous confusing inconsistencies. First, there are a plethora of single-nucleotide differences among the various rabbit 28S and 18S rRNA structures. Second, two nucleotides are absent from the NCBI Reference Sequence for the 18S rRNA gene. Moving forward, we propose using the Broad Institute's rabbit whole-genome shotgun sequence and numbering to reduce modeling ambiguity and improve consistency between ribosome models.
对所有公开可用的（兔）核糖体冷冻电镜结构进行检查后发现了许多令人困惑的不一致之处。首先，在各种兔28S和18S rRNA结构之间存在大量单核苷酸差异。其次，18S rRNA基因的NCBI参考序列中缺少两个核苷酸。展望未来，我们建议使用布罗德研究所的兔全基因组鸟枪法序列和编号，以减少建模的模糊性并提高核糖体模型之间的一致性。
