Zhuang Liumin, Song Chen, Wei Yunru, Han Jinzhi, Ni Li, Ruan Chengxu, Zhang Wen
Institute of Food Science and Technology, College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
Foods. 2025 Feb 26;14(5):795. doi: 10.3390/foods14050795.
This study aimed to investigate the molecular mechanism of with varying adhesion capabilities to 's intestinal mucus influence the spoilage potential of . Sodium chloride(NaCl) was used as an environmental factor to regulate ' adhesion ability. After being exposed to 3.5% NaCl stress, the PS01 strain with low adhesion showed an enhancement in adhesion ability, while the LP-3 strain with high adhesion exhibited a decrease. Correspondingly, the expression of critical adhesion genes, such as , , and , was found to be altered. LP-3, with high adhesion ability, was observed to promote a relative increase in and in fish intestines. This led to the production of more volatile compounds, including 2-octen-1-ol Z, 2,3-Octanedione, and Eicosane, thus deepening the spoilage of tilapia. LP-3, with reduced adhesion ability after NaCl regulation, showed a diminished capacity to cause fish spoilage. Transcriptomics analysis was used to examine two strains that exhibited different adhesion abilities, leading to the identification of an adhesion regulatory network involving flagellar assembly regulation, bacterial chemotaxis, quorum sensing, two-component systems, biofilm formation, and bacterial secretion systems. This study identified the adhesion regulatory pathway and determined 10 key adhesion-related genes.
本研究旨在探究具有不同黏附能力的[具体微生物名称]对[具体鱼类名称]肠道黏液的影响及其对[具体鱼类名称]腐败潜力的分子机制。氯化钠（NaCl）被用作调节[具体微生物名称]黏附能力的环境因子。在受到3.5% NaCl胁迫后，低黏附性的PS01菌株黏附能力增强，而高黏附性的LP - 3菌株黏附能力下降。相应地，发现关键黏附基因如[具体基因名称1]、[具体基因名称2]和[具体基因名称3]的表达发生了改变。高黏附性的LP - 3被观察到促进了[具体鱼类名称]肠道中[具体物质名称1]和[具体物质名称2]的相对增加。这导致产生了更多挥发性化合物，包括Z - 2 - 辛烯 - 1 - 醇、2,3 - 辛二酮和二十烷，从而加深了罗非鱼的腐败程度。经NaCl调节后黏附能力降低的LP - 3导致鱼类腐败的能力减弱。转录组学分析用于检测两种具有不同黏附能力的[具体微生物名称]菌株，从而鉴定出一个涉及鞭毛组装调控、细菌趋化性、群体感应、双组分系统、生物膜形成和细菌分泌系统的黏附调控网络。本研究确定了[具体微生物名称]的黏附调控途径，并确定了10个关键的黏附相关基因。
