Wang Ying, Deng Wengyang, Wu Chaomei, Tian Shihuan, Li Hua
Experimental Center of Preventive Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2025 Mar 20;45(3):488-493. doi: 10.12122/j.issn.1673-4254.2025.03.06.
To investigate how larval feeding regimens influence development and deltamethrin resistance of to provide evidence for standardizing larval feeding protocols in studies of insecticide resistance.
larvae of a laboratory resistant strain were divided into 3 groups (=500) and reared with high, medium, and low food availability (100, 50, or 25 mg daily for the 1st and 2nd instars, and 500 mg 250, or 125 mg daily for 3rd and 4th instars). The developmental time, pupation rate, adult emergence rate, adult body weight, and wing length were recorded in each group, and deltamethrin resistance of the mosquitoes was assessed using larval bioassays and contact tube tests for adults.
Significant developmental differences were observed across the 3 feeding groups. Larval development time decreased as the food availability increased, and both high- and low-food groups showed reduced pupation rates (χ²=16.282, 7.440) and emergence rates (χ²=4.093, 6.977) compared to the medium-food group. Adult body weight and wing length were positively correlated with the amount of larval food intake (<0.05). In high, medium and low food intake groups, larval LC values for deltamethrin were 0.110, 0.072 and 0.064 mg/L, adult KDT50 values were 97.404, 68.964 and 65.005 min, and adult mosquitoe mortality rates at 24 h after deltamethrin exposure were 12%, 16% and 19%, respectively.
The feeding amount during larval stage significantly impacts the development and deltamethrin resistance of , suggesting the importance of standardization of larval nutrition for ensuring comparability of resistance test data across laboratories.
研究幼虫喂养方案如何影响致倦库蚊的发育和对溴氰菊酯的抗性，为杀虫剂抗性研究中幼虫喂养方案的标准化提供依据。
将实验室抗性品系的幼虫分为3组（每组500只），分别用高、中、低食物供应量进行饲养（1龄和2龄幼虫每日分别为100、50或25毫克，3龄和4龄幼虫每日分别为500、250或125毫克）。记录每组的发育时间、化蛹率、成虫羽化率、成虫体重和翅长，并通过幼虫生物测定和成虫接触管试验评估蚊子对溴氰菊酯的抗性。
在3个喂养组中观察到显著的发育差异。随着食物供应量的增加，幼虫发育时间缩短，与中等食物组相比，高食物组和低食物组的化蛹率（χ²=16.282，7.440）和羽化率（χ²=4.093，6.977）均降低。成虫体重和翅长与幼虫食物摄入量呈正相关（P<0.05）。在高、中、低食物摄入量组中，溴氰菊酯的幼虫LC值分别为0.110、0.072和0.064毫克/升，成虫KDT50值分别为97.404、68.964和65.005分钟，溴氰菊酯暴露24小时后成虫死亡率分别为12%、16%和19%。
幼虫阶段的喂养量显著影响致倦库蚊的发育和对溴氰菊酯的抗性，表明标准化幼虫营养对于确保不同实验室抗性测试数据的可比性具有重要意义。
